Trusted by Leading Research & Pharma Institutions

Custom Protein Engineering Services

Transform your protein optimization workflow with precision engineering solutions. From site-directed mutagenesis to directed evolution, we deliver expertly designed protein variants with verified functionality to accelerate your research from concept to discovery.

100% Sequence Verified

AI-Guided Design

Expert Consultation

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Trusted by leading research and pharmaceutical institutions

MIT

Pfizer

Stanford

Novartis

Johns Hopkins

Roche

Why Choose Us

ML-guided mutagenesis design

Deep mutational scanning capability

Constructs up to 15 kb

Comprehensive functional QC

Site-Directed Mutagenesis

Point, insertion, deletion mutations

Saturation Mutagenesis

NNK/NSC codon libraries

Directed Evolution

Error-prone PCR, shuffling

Success Rate

99.8%

Service Overview

Precision Protein Engineering for Every Project

Our comprehensive protein engineering platform combines cutting-edge mutagenesis technologies with AI-guided design to deliver precisely engineered protein variants for your research needs.

Site-Directed Mutagenesis

Precision mutations including point mutations, insertions, and deletions. Achieve 100% sequence accuracy with our optimized protocols and advanced verification.

Single or multi-site mutations
Constructs up to 15 kb
Sanger sequencing verified

Saturation Mutagenesis

Explore comprehensive sequence space with NNK/NSC codon libraries. Perfect for identifying key residues and optimizing protein function.

Complete amino acid coverage
Focused or genome-wide libraries
High-throughput screening

Directed Evolution

Evolve proteins with improved properties through iterative selection. Error-prone PCR and DNA shuffling for novel function discovery.

Error-prone PCR libraries
DNA family shuffling
Multi-round selection

Our Technology

Advanced Engineering Platform

Our platform integrates machine learning-guided design with high-fidelity DNA synthesis to deliver superior protein engineering outcomes.

AI-Guided Mutagenesis Design

Our proprietary algorithms analyze protein structures and sequences to predict optimal mutation sites, reducing experimental iterations and increasing success rates.

High-Fidelity DNA Synthesis

Industry-leading synthesis accuracy ensures your mutations are introduced precisely as designed, with verified sequence integrity across entire constructs.

Deep Mutational Scanning

Comprehensive library generation and screening capabilities enable systematic exploration of sequence space for optimized protein properties.

Multi-Level QC Verification

Every variant undergoes Sanger sequencing and functional validation, ensuring reliability and reproducibility in your downstream applications.

Technology Comparison

Synthesis Fidelity 99.9%

Mutation Accuracy 100%

Max Construct Size 15 kb

Library Diversity 10^8+ variants

Functional QC Included

Service Specifications

Detailed Service Parameters

Service Type	Size Range	Mutation Types	Delivery Format
Site-Directed Mutagenesis	Up to 15 kb	Point, Insertion, Deletion	Lyophilized plasmid, 4-5 μg
Saturation Mutagenesis	NNK/NSC codons	Single position, Multi-position	Library pool or individual clones
Error-Prone PCR Library	Up to 5 kb	Random mutations (0.1-2%)	PCR product or cloned
DNA Shuffling	Multiple gene fragments	Recombination-based	Library or validated variants
Combinatorial Libraries	Up to 10^8 diversity	Custom degenerate positions	Linear or cloned library

99.9%

Synthesis Accuracy

15 kb

Max Construct Size

10^8

Library Diversity

100%

Sequence Verified

Service Process

Streamlined Workflow

From initial consultation to final delivery, our optimized workflow ensures efficiency and quality at every step.

Consultation

Project assessment and mutation strategy design with our expert team

Design

AI-guided mutation design and codon optimization

Synthesis

High-fidelity DNA synthesis with quality control

Verification

Sanger sequencing and functional QC validation

Delivery

Complete documentation and plasmid preparation

Applications

Research Applications

Enzyme Optimization

Enhance enzyme properties including catalytic activity, substrate specificity, thermal stability, and pH tolerance through targeted mutagenesis and directed evolution approaches.

Activity improvement through active site mutagenesis
Substrate specificity engineering for novel substrates
Thermostabilization for industrial conditions
Directed evolution for novel functions

10x

Activity Increase

100+

Variants Screened

50°C

Thermal Stability

48h

Turnaround Time

Testimonials

What Our Clients Say

"The precision of the mutagenesis service exceeded our expectations. Every variant was exactly as designed, saving us significant time in validation."

Research Director

Biotechnology Company

"Their directed evolution platform helped us evolve an enzyme with 50-fold improved activity. The workflow was efficient and well-documented."

Principal Scientist

Pharmaceutical Company

"Excellent technical support throughout our antibody humanization project. The AI-guided design approach really accelerated our timeline."

Senior Scientist

Academic Research Institution

Scientific Literature

Supporting Research

Our services are built on foundational research in protein engineering and directed evolution.

892 Citations

Directed Evolution: Methodologies and Applications

Wang Y, Xue P, Cao M, Yu T, Lane ST, Zhao H. Chemical Reviews, 2021

Comprehensive review of gene diversification strategies, screening/selection methods, and continuous evolution strategies for directed evolution in protein engineering.

View DOI

485 Citations

Low-N protein engineering with data-efficient deep learning

Biswas S, Khimulya G, Alley EC, Esvelt KM, Church GM. Nature Methods, 2021

ML-guided paradigm that uses as few as 24 functionally assayed mutant sequences to build accurate virtual fitness landscapes for protein optimization.

View DOI

127 Citations

Opportunities and Challenges for Machine Learning-Assisted Enzyme Engineering

Yang J, Li FZ, Arnold FH. ACS Central Science, 2024

Review of how ML models complement empirical directed evolution processes for enzyme optimization and protein property enhancement.

View DOI

38 Citations

Structure-based design approach to rational site-directed mutagenesis of β-lactoglobulin

Bonarek P, Loch JI, et al. Journal of Structural Biology, 2020

Demonstration of rational mutagenesis design using 3D structural information to modify binding pocket properties and improve protein stability.

View DOI

187 Citations

Machine learning to navigate fitness landscapes for protein engineering

Freschlin CR, Fahlberg SA, Romero PA. Current Opinion in Biotechnology, 2022

Review of ML approaches for modeling sequence-function relationships and efficiently searching protein sequence space.

View DOI

FAQ

Frequently Asked Questions

What is the definition of a single mutation in site-directed mutagenesis?

A single mutation is defined as any combination of base changes within a 30-base frame. Multiple mutations within 30bp are counted as one mutation. This applies to point mutations, insertions, and deletions within this range.

What is the maximum construct size you can handle?

We can handle constructs up to 15 kb for site-directed mutagenesis. For larger constructs or complex projects, please contact us for a custom consultation to discuss feasibility and timelines.

What quality control methods do you use?

Every variant undergoes comprehensive quality control including Sanger sequencing of the entire mutated region, restriction digest verification (where applicable), and optional functional assay validation. We guarantee 100% sequence accuracy for all delivered constructs.

Do you offer codon optimization services?

Yes, we provide codon optimization using proprietary algorithms to enhance expression in your target host system. This includes E. coli, yeast, and mammalian cells. Optimization considers codon usage bias, GC content, mRNA stability, and elimination of不利 sequence motifs.

What are the options for saturation mutagenesis libraries?

We offer NNK, NNS, and NSC codon options for saturation mutagenesis. Libraries can be delivered as pooled DNA or as individual colony-picked variants. We also provide optional cloning into expression vectors and high-throughput screening services.

Can you handle confidential projects?

Absolutely. We maintain strict confidentiality for all projects. All work is performed following comprehensive data protection protocols, and we can execute confidentiality agreements (NDAs) as required. Sequence information is never shared or published without explicit consent.

What formats can you deliver the final products in?

We offer multiple delivery formats: lyophilized plasmid DNA in tubes or 96/384-well plates, plasmid in solution, glycerol stocks, and transformation-competent cells. All deliveries include comprehensive documentation including sequence chromatograms, construct maps, and COA.

Do you provide functional validation services?

Yes, we offer optional functional validation including protein expression analysis, enzyme activity assays, binding kinetics determination, and thermal stability measurements. These services can be bundled with mutagenesis projects for comprehensive characterization.

Still have questions? We're here to help.

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Custom Protein Engineering Services

Why Choose Us

Site-Directed Mutagenesis

Saturation Mutagenesis

Directed Evolution

Precision Protein Engineering for Every Project

Site-Directed Mutagenesis

Saturation Mutagenesis

Directed Evolution

Ready to Engineer Your Protein?

Advanced Engineering Platform

AI-Guided Mutagenesis Design

High-Fidelity DNA Synthesis

Deep Mutational Scanning

Multi-Level QC Verification

Technology Comparison

Detailed Service Parameters

Streamlined Workflow

Consultation

Design

Synthesis

Verification

Delivery

Research Applications

Enzyme Optimization

Antibody Humanization & Optimization

Key Techniques

Structural Biology Support

Key Techniques

Therapeutic Protein Development

Key Techniques

What Our Clients Say

Supporting Research

Directed Evolution: Methodologies and Applications

Low-N protein engineering with data-efficient deep learning

Opportunities and Challenges for Machine Learning-Assisted Enzyme Engineering

Structure-based design approach to rational site-directed mutagenesis of β-lactoglobulin

Machine learning to navigate fitness landscapes for protein engineering

Frequently Asked Questions

Ready to Start Your Project?

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