Mannheimia succiniciproducens metabolic engineering for succinic acid production

Insights from Literature: Mannheimia succiniciproducens metabolic engineering for succinic acid production

Succinic acid is a 4-carbon dicarboxylic acid. Not only a major intermediate in the TCA, it is also a major raw material in food, pharmaceutical and chemical production. Since the market for sustainable development is increasing, renewable energy for microbial fermentation of succinic acid is gaining traction in the industry. But old-school fermentation tends to be coupled with…

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Improved n-Butanol Production from Clostridium cellulovorans

Insights from Literature: Integrated metabolic and evolutionary engineering to help green production of n-butanol

Driven by the global fossil fuel crisis and environmental issues, the development of biotechnology-based renewable fuels and chemicals has become a research hotspot. As an ideal petroleum alternative, n-butanol has attracted attention due to its superior fuel properties (high energy density, low volatility) and wide range of industrial uses. However, traditional butanol production relies on…

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Biosynthetic pathways of L-malic acid in eukaryotes

Insights from Literature: L-Malate Biosynthesis and Metabolic Engineering Strategies

L-maleic acid is a four carbon dicarboxylic acid found in food, chemical and pharmaceutical industries. L-malic acid is an intermediate in the tricarboxylic acid cycle (TCA), one of the 12 most promising biobased platform chemicals – according to the US Department of Energy. Yet industrial production today mainly depends on chemical production of petrochemical raw materials and…

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Base preferences for CRISPR Cas9 system

Application of CRISPR-Cas9 technology in metabolic engineering

Introduction A brief introduction to CRISPR-Cas9 technology CRISPR-Cas9 technology is a revolutionary gene editing tool based on the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system found in bacteria and archaea. This system originally existed as a defense mechanism against phage infection. CRISPR-Cas9 technology uses Cas9 protein and guide RNA (gRNA) to identify and cut…

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CRISPR/Cas9 genome editing system(Y Cui, et al.,2018)

An in-depth look at CRISPR technology: applications from DNA editing to synthetic biology

CRISPR technology has become the focus of the biotechnology field in recent years, and it has revolutionized the landscape of genetic engineering with its precise, fast and efficient gene editing capabilities. As a revolutionary tool, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has not only accelerated the development of genetic research, but also demonstrated…

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CRISPR/Cas Technology: Revolutionizing Viral Research and Vaccine Development

Viral infections pose a significant global health threat, often leading to chronic diseases and the development of resistance to standard antiviral treatments. CRISPR/Cas technology, initially discovered as part of a bacterial immune defense system, is revolutionizing the way scientists study viruses. This innovative tool allows for the creation of viral mutants, detailed gene studies, and…

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