Glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) are important precursor substances for the synthesis of glycosaminoglycans, with extensive application value in many fields. Microbial fermentation has many advantages in the production of GlcN and GlcNAc, and is increasingly favored. This study constructed genetically engineered strains of Escherichia coli with high production of GlcN and GlcNAc, laying the…
Research background: The biosynthesis of natural products usually requires the binding of eukaryotic cytochrome P450 (P450) to P450 reductase, which is physically close and undergoes electron transfer reactions. Research question: Unfortunately, the functional expression of eukaryotic p450 in bacteria is often still difficult. Research content and results: 1) The author of this article artificially approached…
Theodor Escherich, an outstanding physician and scientist of the 19th century, was one of the earliest scientists to explore microorganisms. He founded the field of intestinal pathology and associated his name with the name Escherichia coli, which was discovered and named in 1885. From then on, the “E.coli clan” was endowed with the fate of…
In 2011, countries such as the United States, China, the United Kingdom, Singapore, and Australia launched the “Sc2.0 Project” to redesign and synthesize all 16 chromosomes of brewing yeast. This was the first attempt by humans to design and synthesize the genome of eukaryotes from scratch. On November 8, 2023, the latest research findings…
Recently, Tosin Thompson published a news article on Nature titled “How CRISPR gene editing could help treat Alzheimer’s”. Alzheimer’s disease is the most common form of dementia, affecting over 55 million people worldwide. It is expected that this number will nearly double by 2050. Most research on Alzheimer’s disease is driven by the amyloid protein…
Less than a month after the world’s first approval of CRISPR-Cas9 genome editing therapy, this therapy received a second approval – this time from the United States, due to its strict regulation and lucrative healthcare market. The UK regulatory agency approved this therapy on November 16th, which inactivates a gene as a means of treating…
Researchers at the Max Planck Institute of Land Microbiology have developed a synthetic biochemical cycle that directly converts carbon dioxide into the core acetyl CoA. Researchers are able to implement each of these three cycle modules in Escherichia coli, which represents an important step in achieving the synthesis of carbon dioxide fixation pathways in a…
Engineering proteins that enhance stability are widely used in the development of biological therapies or diagnostic reagents, as well as in other commercial and industrial applications. For this reason, many related experimental and computational methods have been developed, such as directed evolution, rational design, ancestor sequence reconstruction, and molecular dynamics simulation, which stabilize proteins by…
As is well known, microorganisms can produce abundant secondary metabolites and are an important source for drug development. Streptomyces is a star species in the development and research of microbial drugs, and many medical or agricultural antibiotics such as rapamycin (immunosuppressive agent), nystatin (antifungal), avermectin (insecticidal), and azamycin (antibacterial) are produced from Streptomyces. However, the…
Research background: The use of renewable substrates such as glycerol to produce 3-HP is a promising method. However, the production of 3-HP from glycerol requires vitamin B-12, which hinders its economic feasibility. Research has shown that using glycerol as a substrate β- The alanine pathway can efficiently produce 3-HP. Therefore, a comparison was made between…