Introduction A brief introduction to CRISPR-Cas9 technology CRISPR-Cas9 technology is a revolutionary gene editing tool based on the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system found in bacteria and archaea. This system originally existed as a defense mechanism against phage infection. CRISPR-Cas9 technology uses Cas9 protein and guide RNA (gRNA) to identify and cut…
CRlSPR/Cas9 is an acquired immune system used by bacteria and paleontological bacteria to resist the invasion of genetic material such as foreign plasmids or bacteriophages. The CRlSPR/Cas9 technology was used to establish a mammalian genome-wide mutation library or a gene mutant library related to a certain type of function, and the genes related to the…
Gene editing is an emerging genetic engineering technology that can modify specific target genes in the genome of organisms. Gene editing can efficiently perform site-directed genome editing, so it has shown great application prospects in gene research, gene therapy and gene breeding. To date, CRISPR/Cas9 has been widely used in the scientific community as an…
CRISPR technology has become the focus of the biotechnology field in recent years, and it has revolutionized the landscape of genetic engineering with its precise, fast and efficient gene editing capabilities. As a revolutionary tool, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has not only accelerated the development of genetic research, but also demonstrated…
Viral infections pose a significant global health threat, often leading to chronic diseases and the development of resistance to standard antiviral treatments. CRISPR/Cas technology, initially discovered as part of a bacterial immune defense system, is revolutionizing the way scientists study viruses. This innovative tool allows for the creation of viral mutants, detailed gene studies, and…
The recombinant protein expression technology was established in the early 70s of the 20th century, in which the target gene was introduced into the host cell through a vector, and the target protein was produced with the help of the host’s protein production mechanism. Nowadays, many researchers use prokaryotic or eukaryotic cells such as Escherichia…
Every leap forward in technology is drawing a blueprint for the future. Among the many scientific and technological innovations, biotechnology, with its unique charm and far-reaching impact, is gradually becoming a key force to promote global progress. It has not only revolutionized the healthcare sector, but has also shown its unlimited potential in a variety…
Did you know that “tiny” cells are also ‘factories’? Nowadays, biosynthesis technology with the concept of “green and sustainable development” is playing an increasingly important role. However, the protagonists of this clean revolution are not the factories with tall chimneys, but the micron-sized microorganisms (most of them), known as “cell factories”. Q1 So, how do…
Filamentous fungi, collectively known as molds, are widely used. Widely distributed in nature due to its abundant species, wide sources, and ease of use Due to its characteristics of industrialization, it has been widely applied in multiple fields. song Filamentous species such as Aspergillus and Trichoderma. Fungi have excellent protein synthesis and secretion abilities and…
The rapid development of genome editing technology has enabled precise and efficient genome modification in the field of microorganisms. This technology not only enables scientists to better understand the biological characteristics of microorganisms, but also provides unprecedented possibilities for fields such as biopharmaceuticals, industrial production, and environmental protection. CRISPR-Cas9 is a revolutionary gene editing technology,…