1. Introduction to Codon Optimization Codon optimization represents an influential molecular biology method that modifies a gene’s nucleotide sequence to enhance protein production in a target organism. Scientists achieve improved translation efficiency and protein yield while preserving functional integrity when they match the gene’s codon usage to the host’s translational system. Achieving high expression levels…
The protein engineering and drug discovery domains have undergone transformative changes due to mRNA display and ribosome display technologies which allow scientists to study protein functionality and evolve enzymes while identifying novel therapeutic agents. These techniques allow for the examination of extensive protein and peptide libraries although their operational principles and practical uses show significant…
The field of protein engineering and drug discovery now benefits from mRNA display technology as an effective means to identify proteins and antibodies with high affinity alongside peptides. Custom mRNA display libraries represent the fundamental element of this technology because they enable the identification of functional molecules. This blog post examines the techniques and innovative…
Introduction A brief introduction to CRISPR-Cas9 technology CRISPR-Cas9 technology is a revolutionary gene editing tool based on the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system found in bacteria and archaea. This system originally existed as a defense mechanism against phage infection. CRISPR-Cas9 technology uses Cas9 protein and guide RNA (gRNA) to identify and cut…
CRlSPR/Cas9 is an acquired immune system used by bacteria and paleontological bacteria to resist the invasion of genetic material such as foreign plasmids or bacteriophages. The CRlSPR/Cas9 technology was used to establish a mammalian genome-wide mutation library or a gene mutant library related to a certain type of function, and the genes related to the…
Gene editing is an emerging genetic engineering technology that can modify specific target genes in the genome of organisms. Gene editing can efficiently perform site-directed genome editing, so it has shown great application prospects in gene research, gene therapy and gene breeding. To date, CRISPR/Cas9 has been widely used in the scientific community as an…
CRISPR technology has become the focus of the biotechnology field in recent years, and it has revolutionized the landscape of genetic engineering with its precise, fast and efficient gene editing capabilities. As a revolutionary tool, CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology has not only accelerated the development of genetic research, but also demonstrated…
Viral infections pose a significant global health threat, often leading to chronic diseases and the development of resistance to standard antiviral treatments. CRISPR/Cas technology, initially discovered as part of a bacterial immune defense system, is revolutionizing the way scientists study viruses. This innovative tool allows for the creation of viral mutants, detailed gene studies, and…
The recombinant protein expression technology was established in the early 70s of the 20th century, in which the target gene was introduced into the host cell through a vector, and the target protein was produced with the help of the host’s protein production mechanism. Nowadays, many researchers use prokaryotic or eukaryotic cells such as Escherichia…
Every leap forward in technology is drawing a blueprint for the future. Among the many scientific and technological innovations, biotechnology, with its unique charm and far-reaching impact, is gradually becoming a key force to promote global progress. It has not only revolutionized the healthcare sector, but has also shown its unlimited potential in a variety…