Did you know that “tiny” cells are also ‘factories’? Nowadays, biosynthesis technology with the concept of “green and sustainable development” is playing an increasingly important role. However, the protagonists of this clean revolution are not the factories with tall chimneys, but the micron-sized microorganisms (most of them), known as “cell factories”. Q1 So, how do…
Filamentous fungi, collectively known as molds, are widely used. Widely distributed in nature due to its abundant species, wide sources, and ease of use Due to its characteristics of industrialization, it has been widely applied in multiple fields. song Filamentous species such as Aspergillus and Trichoderma. Fungi have excellent protein synthesis and secretion abilities and…
The rapid development of genome editing technology has enabled precise and efficient genome modification in the field of microorganisms. This technology not only enables scientists to better understand the biological characteristics of microorganisms, but also provides unprecedented possibilities for fields such as biopharmaceuticals, industrial production, and environmental protection. CRISPR-Cas9 is a revolutionary gene editing technology,…
In the biopharmaceutical process, cell lines play a crucial role as a marker for the activation of CMC. A good cell line can not only reduce the difficulty of CMC development, but also greatly reduce the cost of drug development. However, the development of cell lines is a long and complex process, with multiple stages…
What is miRNA MiRNA generally refers to micro RNA. MicroRNAs (miRNAs) are a class of non coding single stranded RNA molecules with a length of approximately 19-24nt encoded by endogenous genes, which participate in post transcriptional gene expression regulation in animals and plants. MiRNAs are usually located between genes or in intronic regions, so it…
Metabolic engineering is a powerful field that combines the principles of biology, chemistry, and engineering to optimize genetic and regulatory processes within cells. This process aims to enhance the production of specific substances. E.coli, a model organism with well-characterized genetics, rapid growth, and versatility, plays a significant role in metabolic engineering. Let’s explore the impact…
In cells, DNA often exists in the form of double stranded helices, and in its active state, it may appear in a single stranded form, participating in biological processes such as replication and transcription. SsDNA (single stranded DNA), also known as single stranded DNA, can be used as a template for homology directed repair (HDR)…
The function of proteins is programmed and determined by the genetic information of organisms, which have evolved in nature. However, the characteristics formed during natural selection and evolution sometimes do not meet the strict standards and conditions required in industrial production or medical applications. For example, insufficient protein stability in extreme industrial environments, or direct…
April 25th is DNA day, a big day in the history of biology, because 68 years ago, on April 25th, 1953, James Watson, Francis Crick, and their colleagues published a paper on the double helix structure of DNA in Nature. Since then, the mystery of DNA structure that has plagued generations has been solved, and…
Plasmid DNA is a small double stranded circular DNA molecule that is exposed (without protein binding), structurally simple, independent of bacterial nucleoid DNA, and has the ability to self replicate. Image plasmids usually carry only a small number of genes, especially those related to antibiotic resistance; In addition, plasmids can be transmitted between different bacterial…