Plasmid DNA is a small double stranded circular DNA molecule that is exposed (without protein binding), structurally simple, independent of bacterial nucleoid DNA, and has the ability to self replicate. Image plasmids usually carry only a small number of genes, especially those related to antibiotic resistance; In addition, plasmids can be transmitted between different bacterial…
1、 DNA source The double stranded length of DNA is commonly described in units of bp, Kb, Mb, and Gb. 1bp=one base pair; 1kb=1000bp; 1Mb=1000kb; 1Gb=1000Mb. The length of a single stranded DNA is represented by nt, where 10nt=10 base lengths of a single stranded DNA. 1. Genomic DNA (gDNA) Eukaryotes, double stranded linear DNA…
In recent years, CRISPR gene editing technology has attracted great attention due to its enormous potential in the treatment of hereditary diseases. However, the potential off target effects that may occur during gene editing have always been an important safety concern. In addition, different populations have different genetic information, which poses great difficulties in accurately…
CRISPR gene editing is widely recognized as the most significant and groundbreaking breakthrough in life science since the 21st century. It was officially born in 2012 and was recognized by the Nobel Prize in just 8 years. At the end of last year, the first CRISPR based gene editing therapy was approved by the FDA…
01 FDA approved new gene editing therapy for the treatment of OTC deficiency in clinical practice On April 4, 2024, the FDA approved the clinical study approval application for ECUR-506 for the treatment of neonatal patients with ornithine aminotransferase (OTC) deficiency. OTC deficiency, also known as hyperammonemia type II, is an X-linked genetic disease caused…
Colorectal cancer (CRC) is one of the main cancers that threaten life and health, causing a serious social burden. Long term use of NSAIDs can significantly reduce its incidence rate, but it can not be completely prevented and treated. Resection is an early intervention measure that can be combined with chemotherapy and radiation therapy, but…
Abstract Hydrogen sulfide (H2S) is a gaseous microbial metabolite, and its role in intestinal diseases is highly controversial. This is mainly due to the difficulty of accurately measuring H2S and the use of model systems that cannot accurately represent the human intestinal environment for research. In this paper, the engineered Escherichia coli designed by the…
Many microorganisms accumulate compatible solutes to counteract external pressures such as high osmotic pressure, high temperature, drying, and freezing. Among numerous compatible solutes, tetrahydropyrimidine stands out for its significant efficacy in effectively protecting halophilic cells from osmotic pressure imbalance and maintaining the stability of intracellular biomolecules. Recent studies have shown that tetrahydropyrimidine has the potential…
Metabolic engineering refers to the purposeful modification and modification of cellular metabolic pathways using genetic engineering techniques, altering cellular characteristics, and producing specific target products. Metabolic engineering has a history of 30 years since its development. From traditional modification techniques such as knocking out byproduct generation pathways, releasing product synthesis inhibition, and overexpressing key enzymes…
According to a new study by researchers at the University of Michigan, a protein engineering method using simple, cost-effective experiments and machine learning models can predict which proteins are effective for specific purposes. This method has profound potential in assembling proteins and peptides, and can be used for applications ranging from industrial tools to therapeutic…