As an important component of mammalian cell expression system, CHO cells can be genetically engineered to optimize their metabolic pathways, improve the expression efficiency of exogenous proteins, enhance their adaptability and stability, and provide strong support for the research and production of biopharmaceutical products. In the previous issue, we introduced commonly used methods for genetic…
Research background Lutein is a key carotenoid pigment used in plant photosynthesis, and it is also crucial for maintaining human health. For example, lutein has a protective effect on the eyes and also has antioxidant properties, inhibiting the damage of oxygen free radicals to cells. However, due to the abundance of related coding genes, there…
Highlights Metabolic flux provides quantitative insights into carbon, energy, and electron flow. Flux Balance Analysis (FBA) evaluates the ability of metabolic network models. 13C Metabolic flux analysis (13C MFA) is used to estimate flux with high precision. The new method of 13C-MFA can measure flux in complex, non-standard biological systems. The predictive kinetic model of…
CRISPR interference (CRISPRi) is a system based on CRISPR. This editing allows ribonucleoprotein (RNP) complexes to be directed and bind to target DNA, interfering with RNA polymerase (RNAP) to inhibit transcription initiation or elongation. Unlike the CRISPR/Cas9 system, CRISPRi allows for a reduction in gene expression by expressing dCas9 or sgRNA under inducible promoters, thereby…
Proteins are one of the most important molecules in living organisms, with an extremely wide range of functions and roles. Traditionally, researchers can use cell expression systems to prepare the required proteins and conduct in-depth research on them. However, due to factors such as cell growth, maintenance, and contamination, the cell expression system faces some…
The emergence of CRISPR-Cas9 gene editing technology has significantly changed the landscape of gene editing, providing scientists with unprecedented convenience and efficiency in freely manipulating genes. However, the accuracy of CRISPR gene editing is crucial for ensuring clinical safety and avoiding accidental and permanent genotoxicity. In recent years, CRISPR-Cas9 gene editing has made advanced T…
Overview In November of this year, the Medicines and Health Products Regulatory Authority (MHRA) in the UK approved a CRISPR/Cas9 gene editing therapy for sickle cell disease, which caused quite a sensation at the time. It should be noted that this was the world’s first approved CRISPR therapy. Although CRISPR technology has been highly sought…
Prime Editing is another technology developed by Professor David R. Liu’s team at Harvard University for precise gene editing, following Base Editing. The development of PE perfectly solves the problem of being able to effectively repair only four gene mutations (i.e. C → T, G → A, A → G, and T → C) in…
Since the intensive progress in the field of gene editing therapy was released, it has to some extent boosted confidence in the industry. The attitude and actions of the FDA are key factors in promoting the commercialization of gene editing therapy. Since Feng Zhang first demonstrated in 2013 that CRISPR-SpCas9 can successfully perform gene editing…
When it comes to molecular cloning, almost every friend who has done plasmid construction knows about Gibson Assembly, and this method is really popular! I remember when I first entered the laboratory, my senior brothers and sisters taught me that the method used to construct plasmids was Gibson Assembly. At that time, the entire laboratory…