Curcumin is a polyphenolic compound with good biological activity. It exists in the plant turmeric and has low water solubility. It has great potential as an antioxidant, anti-inflammatory, anti-cancer and anti diabetes drug. Therefore, curcumin is considered a safe and promising drug that can be used to prevent and treat various diseases. Curcumin is mainly…
1、 Phenylalanine metabolism Phenylalanine (PA) has racemic DL type, L type, and D type. L-type is an essential amino acid for humans and animals, with a molecular weight of 165.2. It belongs to the aromatic amino acid group and mainly participates in the composition of various protein components, which can only be obtained from food….
Introduction: In the vast palette of nature’s creations, melanin stands out as a pigment of remarkable complexity and importance. Found in varying concentrations across living organisms, melanin plays diverse roles, from determining skin color to protecting against harmful UV radiation. This article aims to delve into the fascinating world of melanin, exploring its structure, functions,…
Malonic acid is an important intermediate, and its derivatives have important applications in the fields of medicine, agriculture, chemical industry, and food processing. It can be expressed in Escherichia coli through β- The alanine pathway achieves biosynthesis, with a yield of 3.6 g/L. Fu Wenxuan and others followed the path of phosphoenolpyruvate/pyruvate → oxaloacetic acid…
As an important component of mammalian cell expression system, CHO cells can be genetically engineered to optimize their metabolic pathways, improve the expression efficiency of exogenous proteins, enhance their adaptability and stability, and provide strong support for the research and production of biopharmaceutical products. In the previous issue, we introduced commonly used methods for genetic…
Research background Lutein is a key carotenoid pigment used in plant photosynthesis, and it is also crucial for maintaining human health. For example, lutein has a protective effect on the eyes and also has antioxidant properties, inhibiting the damage of oxygen free radicals to cells. However, due to the abundance of related coding genes, there…
Highlights Metabolic flux provides quantitative insights into carbon, energy, and electron flow. Flux Balance Analysis (FBA) evaluates the ability of metabolic network models. 13C Metabolic flux analysis (13C MFA) is used to estimate flux with high precision. The new method of 13C-MFA can measure flux in complex, non-standard biological systems. The predictive kinetic model of…
CRISPR interference (CRISPRi) is a system based on CRISPR. This editing allows ribonucleoprotein (RNP) complexes to be directed and bind to target DNA, interfering with RNA polymerase (RNAP) to inhibit transcription initiation or elongation. Unlike the CRISPR/Cas9 system, CRISPRi allows for a reduction in gene expression by expressing dCas9 or sgRNA under inducible promoters, thereby…
Proteins are one of the most important molecules in living organisms, with an extremely wide range of functions and roles. Traditionally, researchers can use cell expression systems to prepare the required proteins and conduct in-depth research on them. However, due to factors such as cell growth, maintenance, and contamination, the cell expression system faces some…
The emergence of CRISPR-Cas9 gene editing technology has significantly changed the landscape of gene editing, providing scientists with unprecedented convenience and efficiency in freely manipulating genes. However, the accuracy of CRISPR gene editing is crucial for ensuring clinical safety and avoiding accidental and permanent genotoxicity. In recent years, CRISPR-Cas9 gene editing has made advanced T…