Overview In November of this year, the Medicines and Health Products Regulatory Authority (MHRA) in the UK approved a CRISPR/Cas9 gene editing therapy for sickle cell disease, which caused quite a sensation at the time. It should be noted that this was the world’s first approved CRISPR therapy. Although CRISPR technology has been highly sought…
Prime Editing is another technology developed by Professor David R. Liu’s team at Harvard University for precise gene editing, following Base Editing. The development of PE perfectly solves the problem of being able to effectively repair only four gene mutations (i.e. C → T, G → A, A → G, and T → C) in…
Since the intensive progress in the field of gene editing therapy was released, it has to some extent boosted confidence in the industry. The attitude and actions of the FDA are key factors in promoting the commercialization of gene editing therapy. Since Feng Zhang first demonstrated in 2013 that CRISPR-SpCas9 can successfully perform gene editing…
When it comes to molecular cloning, almost every friend who has done plasmid construction knows about Gibson Assembly, and this method is really popular! I remember when I first entered the laboratory, my senior brothers and sisters taught me that the method used to construct plasmids was Gibson Assembly. At that time, the entire laboratory…
Glucose metabolism refers to all processes involved in breaking down or re forming glucose. Glycolysis is the process of breaking down glucose into molecules such as pyruvate and ATP. It occurs within the cytoplasm and does not require oxygen. This makes glucose an anaerobic process. In the absence of oxygen, the pyruvate molecules formed during…
MRNA (messenger RNA) is an important molecular biology messenger that can carry genetic information within cells, converting genetic information in DNA into instructions for protein synthesis. In recent years, mRNA technology has received increasing attention in the medical field, especially in vaccine development and gene therapy. With the rapid development of genomics and proteomics, people’s…
In the realm of protein research, few names resonate as deeply as that of David Baker. His significant work has effectively sculpted our understanding of proteins, their complex structures, and their multifaceted functions. This blog aims to delve into Baker’s remarkable contributions, providing an in-depth exploration of his work on protein structure prediction, protein design,…
In the rich and multifaceted realm of biology, there exist two crucial terms that every student, researcher, and biology enthusiast must comprehend – “phenotype” and “genotype”. These twin concepts form the very foundation of genetics, playing a significant role in explaining how traits are passed down from parents to their offspring. This blog post aims…
Introduction The world of non-coding RNAs has sparked considerable interest within the scientific community due to their influential role in the regulation of gene expression. Among these non-coding RNAs, siRNA (small interfering RNA) and miRNA (microRNA) have stood out, attracting significant attention from researchers worldwide. Both these RNA molecules are involved in an important biological…
Glucosamine (GlcN) and N-acetylglucosamine (GlcNAc) are important precursor substances for the synthesis of glycosaminoglycans, with extensive application value in many fields. Microbial fermentation has many advantages in the production of GlcN and GlcNAc, and is increasingly favored. This study constructed genetically engineered strains of Escherichia coli with high production of GlcN and GlcNAc, laying the…