Effective Degradation of Antibiotics by Laccase Based Self Amplifying Catalytic System for Sustainable Environmental Remediation

The accumulation of antibiotics in agricultural soils poses a potential threat to soil ecosystems and crop growth, ultimately posing a threat to human health. Tetracycline antibiotics (TCs), such as tetracycline (CTC), oxytetracycline (OTC), and tetracycline (TTC), are widely present in soil as antibacterial agents. Generally speaking, tetracycline is difficult to completely metabolize in the body,…

Read More

Multiple aspects of sulfur incorporation into natural product biosynthesis

Sulfur-containing natural products (sulfur-containing NPs) cover almost every type of NP discovered so far. The source of sulfur in peptide NPs is usually composed of cysteine and methionine. Methionine can be directly incorporated into peptide NPs without any modification, such as precarriebowamide (1). The cysteine residues in peptide NPs are typically embedded in intramolecular disulfides,…

Read More

Spanish researchers have developed the smallest amiRNA that can reduce the cost of RNA pesticides and pave the way for large-scale production

RNA interference (RNAi) based biopesticides are considered a disruptive technology in the field of plant protection in the future, which will greatly change human thinking and strategies for controlling harmful organisms such as agricultural diseases, insects, and grasses. Although it has not yet been widely used, the cost of RNA production is high, and the…

Read More

Plant Promoters and Terminators in High Precision Bioengineering

High precision bioengineering and synthetic biology require fine-tuning of gene expression at the transcriptional and post transcriptional levels, and gene transcription itself is strictly regulated by promoters and terminators. Promoters include core promoters and distal promoters (Figure 1), which can determine the practice, organization, and cellular level of gene expression. The terminator is inserted into…

Read More

Amyris: kilogram level GMP synthesis of semi synthetic vaccine grade squalene

Since the 1990s, the lotion of triterpene squalene ((6E, 10E, 14E, 18E) -2,6,10,15,19,23-hexamethyl-tetraeicosane -2,6,10,14,18,22-hexene, CAS 111-02-4) has been used as an adjuvant of influenza vaccine. Other indications such as tuberculosis, malaria, schistosomiasis and leishmaniasis are being evaluated clinically. Except for aluminum oxide salts, squalene based emulsions are the most commonly used vaccine adjuvants in licensed…

Read More

Metabolic engineering transformation of lipolytic Yeast produces a high yield of 17.6 g/L lycopene

Introduction   Compared with methods such as chemical synthesis, using engineering microorganisms to produce high value-added products is a very attractive approach. However, when long and complex compound synthesis pathways are introduced into cells, such as isoprene like compounds, cells activate some regulatory mechanisms to respond to changes in the external environment and achieve their…

Read More

Synthesis of plant natural product precursor p-coumaric acid by Corynebacterium glutamicum

Research background:   Phenylpropanoid compounds such as p-CA are important precursors for the synthesis of various plant secondary metabolites, including stilbenes, flavonoids, and lignans. These compounds have pharmacological significance due to their beneficial health properties. Although it is feasible to extract or chemically synthesize p-coumaric acid from plant raw materials, the use of glucose for…

Read More
Selecting improved variants from heavily-skewed populations

High screening of non classical amino acid enzyme activity through the ability of coupling essential genes to resist termination codons

In enzyme engineering, life and death selection allows for the rapid and direct identification of efficient biocatalysts from a large library. However, the evolution of biocatalysts (related to industry) based on life and death selection is challenging as they require highly reliable strategies to artificially link their activity to host survival.   Recently, Clemens Mayer…

Read More
A synthetic P. putida consortium for simultaneous TPA and EG degradation.

Sustainable upgrading of plastics to biodegradable polymers in a synthetic microbial ecosystem

A synthetic P. putida consortium for simultaneous TPA and EG degradation. In response to the astonishing growth of global plastic pollution, researchers have developed a synthetic microbial ecosystem that can collectively upgrade plastics to the required chemicals. Researchers did not attempt to synthesize a single organism that could complete all the upgrading and recycling steps, but instead…

Read More