Automated HTP Enzyme Assay Development

CD Biosynsis is a leader in the field of enzyme evolution and design. We have a professional team of scientists with a long-term commitment to providing targeted enzyme evolution and design services to researchers. Our EnzymoGeniusTM automates the dispensing of samples and reagents accurately and quickly, ensuring consistent and reproducible results, minimizing human error, and increasing assay throughput. In short, our EnzymoGeniusTM platform aims to provide customers with automated, high-throughput enzyme assays based on their unique customization requirements.

Overview

In the traditional enzyme screening process, it is usually necessary to test variants of a single enzyme one by one. This method has high accuracy, but the detection throughput is also reduced. For the large number of enzyme mutation libraries, it is crucial to find high throughput and automated enzyme detection methods. With the advantages of low sample consumption, high sensitivity and automated analysis, microfluidics is well suited for high-throughput rapid screening and characterization of enzymes. Droplet-based microfluidic high-throughput screening methods offer automation advantages and can simplify the traditional screening process. Currently, droplet-based microfluidic systems are widely used in the biomedical field, bringing potential opportunities for molecular diagnostics of diseases, and enzyme design and optimization.

Fig. 1 Examples of droplet manipulation. (Trinh TND, et al., 2023)

Our Services

The EnzymoGenius™ platform offers automated droplet microfluidic chip development services, providing professional technical consultation and complete solutions to meet customers' needs for automated and efficient screening of target enzymes.

The general manufacturing development process for automated droplet microfluidic chips is shown in the following flowchart.

Advantages of Our Services

• Shorten the Reaction Time: The high surface area to volume ratio of the microfluidic system provides the advantage of shortening the reaction time for enzyme activity assay and screening, which can efficiently meet the requirements of enzyme high-throughput screening processes.
• Provide Multiple Assays: Droplet-based microfluidic devices provide multiple assays for high-throughput enzyme screening. Among the current microfluidic chip detection technologies, optical and electrochemical detection methods are most widely used.
• Provide Controlled Environment: The droplet-based microfluidic system provides a controlled environment for expression vectors for various types of enzyme tests such as bacteria and cells, allowing the enzyme screening process to be minimally interfered with by environmental factors.
• Easy Observation of Results: The system allows isolation of individual cells and simple observation of the reaction of individual enzymes or genes in expression vectors, and can meet the different requirements of different screening systems for screening systems.

With our extensive experience and advanced in silico platform, CD Biosynsis is confident in providing the best enzyme automated high throughput screening assay development services to clients worldwide. We guarantee the most satisfactory results helping to accelerate your project development process. If you are interested in our services, please contact us now for your exclusive solution.

Reference

1. Trinh, TND.; et al. Droplet-Based Microfluidics: Applications in Pharmaceuticals. Pharmaceuticals. 2023, 16(7):937.