De Novo Enzyme Design Service

De Novo Enzyme Design is a ground-breaking tool in the field of biochemistry, and it is rapidly emerging as a game changer in a variety of industries. This innovative process involves the design and creation of new enzymes, which are proteins that catalyze biochemical reactions, with customized properties to perform specific, non-natural chemical reactions.

Structures of designed enzymes(L Jiang, et al., 2008)

By leveraging the power of De Novo Enzyme Design, we can engineer new enzymes that do not exist in nature, thereby opening up a whole new world of possibilities in biochemical reactions. This revolutionary technique has the potential to transform industries by allowing for more efficient processes, cost reductions, and more sustainable production methods.

Technical Route

Our approach to De Novo Enzyme Design is systematic and rigorous. It begins with the identification of a desired chemical reaction that you, our client, wants to catalyze. Our team of scientists and researchers then design an enzyme active site, which is the region of the enzyme where substrate molecules bind and undergo a chemical reaction.

Utilizing advanced computational methods and aided by state-of-the-art technology, we create a protein fold that can house the active site. This is a critical step in ensuring the efficiency and efficacy of the enzyme.

The resulting enzyme design, a perfect blend of science, technology, and innovation, is then synthesized in our lab. We utilize top-tier synthesis methods to ensure the highest quality of enzymes.

After synthesis, the enzyme is rigorously tested for activity to ensure that it performs as expected, providing you with the most efficient catalyst for your specific reaction.

Service Process

Our service process is designed to be client-centric and transparent:

1. Consultation: We begin by understanding your needs. Our team of experts engages in in-depth discussions with you to understand the chemical reaction you desire and the specific requirements you have.
2. Design: Based on our consultation, we design a customized enzyme to facilitate your desired reaction. Our designs are backed by rigorous scientific research and advanced computational methods.
3. Synthesis: Once the design is finalized, the enzyme is synthesized in our state-of-the-art lab. We use cutting-edge technology and the latest scientific methods to ensure the enzyme is synthesized accurately.
4. Testing: To ensure that you receive a product that meets your expectations, the enzyme is rigorously tested for activity and efficiency. We adhere to strict quality control measures to ensure the highest standards.
5. Delivery: Once the enzyme has passed all tests and quality checks, the final product is delivered to you, ready for use in your desired application.

For more information or to discuss a potential project, please feel free to contact us. Our team of experts is ready to assist you and discuss how our De Novo Enzyme Design services can meet your needs.

Case Study

Catalytic activity of G4-DFtet and variants. (A) Oxidation of 4AP catalyzed by variants of DFtet AaAbB2 and DFtet (5 M tetramer). The last four bars in the histogram are for DFtet-A2B2 (in bold). (B) Catalysis of the oxidation of 4AP by G4-DFtet (squares, 100 M) and L2G2-DFtet (diamonds, 100 M) and the background reaction (triangles). (C) Rate of oxidation of 4AP vs. substrate concentration for G4-DFtet (5 M tetramer). (D) Competitive inhibition of the oxidation of 4AP by 4CP (5M tetramer; 0.5 mM). All reactions included 0.15 M Mops (pH 7.0), 0.15 M NaCl, 0.5 mM 4AP, 10 mM MPD, and reactions in A, C, and D included 0.5 mM 4AP.