Design and Optimization of Engineered Enzymes for Biosensors

Our advanced enzyme AI design platform - EnzymoGenius™ specializes in the design and delivery of custom-engineered enzymes tailored for biosensors in the domain of diagnostics research applications.  Leveraging cutting-edge AI technologies, we offer a comprehensive suite of services that empower researchers to craft precise and efficient enzyme solutions.

Background

Engineered enzymes play crucial roles in the development of biosensors. A few significant engineered enzymes include glucose oxidase, horseradish peroxidase, and alkaline phosphatase. Glucose oxidase, derived from microorganisms, is commonly used in glucose monitoring for diabetes management. It changes glucose into gluconolactone and hydrogen peroxide, aiding in the detection process. Horseradish peroxidase, usually mentioned with HRP, serves in enzyme immunoassay techniques, capitalizing on its ability to amplify the signals, and fostering high sensitivity for detecting low concentrations of various substances. Alkaline phosphatase, a hydrolase enzyme, gets exploited in numerous areas of diagnostics and immunological research due to its exceptional stability and broad substrate specificity.

Fig 1. Major biosensing elements and their mechanisms of action. (Alvarado R L, et al., 2021)

The engineering of enzymes involves alterations of the genetic and molecular structures to cater to specific needs. Enhancements usually focus on increasing sensitivity, specificity, or stability to external factors such as pH or temperature, often achieved through methods like directed evolution or rational design. The applicative versatility of these engineered enzymes remains pivotal in fortifying the capabilities of biosensor technology.

Products and Solutions Offering

Applications in Diagnostic Research

• Infectious Diseases. Engineered enzymes find utility in rapid pathogen detection.
• Cancer Biomarkers. Enzyme-based biosensors aid in cancer marker analysis.
• Neurodegenerative Diseases. Precise enzymatic reactions support neurodegenerative disease research.
• Cardiovascular Disorders. Custom enzymes enable cardiovascular biomarker detection.
• Metabolic Diseases. Engineered enzymes contribute to metabolic disorder diagnosis.
• Autoimmune Conditions. Enzyme-driven biosensors assist in autoimmune disease studies.

Technical Advantages

Our custom-engineered enzymes, fortified with cutting-edge technologies, serve as invaluable tools for researchers exploring various disease applications. Whether you require engineered enzymes, innovative technologies, or customized biosensor applications, CD Biosynsis is your trusted partner in advancing the boundaries of diagnostic research. For bespoke solutions, technical expertise, and transformative technologies, we invite you to contact us.

Reference

1. Alvarado, R. L.; et al. Enzyme (Single and Multiple) and Nanozyme Biosensors: Recent Developments and Their Novel Applications in the Water-Food-Health Nexus. Biosensors. 2021, 11(11): 410.