CD Biosynsis is a leading research organization in the field of enzyme design and evolution, with long and in-depth experience in directed enzyme evolution. Our professional research team provides highly-quality services to help researchers improve enzyme-substrate reactivity and provide valuable insights for the rational design of enzymes with better properties or new functions.
Overview
Covalent catalysis is a type of chemical reaction that temporarily forms a covalent bond intermediate between an enzyme and a substrate during an enzymatic reaction. The enzyme active site designed for covalent catalysis aims to create a specific environment within the enzyme to facilitate the formation of covalent bonds between the enzyme and the substrate.
Fig 1. Proposed mechanisms of homogenous catalytic O2 reduction by FeⅡ.(Reed CJ, et al., 2021)
Our Services
Based on our advantages in the field of computer-aided design and AI applications, the EnzymoGenius™ platform aims to provide enzyme active sites designing services for enzyme active sites applied to covalent catalysis.
- Identify Amino Acid Residues
Identify amino acid residues of the enzyme that may potentially participate in covalent catalysis. Common nucleophiles include thiol groups of cysteine, amino groups of lysine, and hydroxyl groups of serine or tyrosine.
- Design Enzyme Active Sites
Introduce more residues into the active site of the enzyme or modify existing residues to create the desired microenvironment to bring the catalytic residues closer to the substrate.
- Computational Modeling Prediction
Some computational prediction methods, such as molecular docking and molecular dynamics modeling, are able to simulate the binding mode of enzymes to substrates, which can help us predict the active site and identify potential catalytic residues of enzymes.
- Site-Directed Mutagenesis
Based on the results predicted by the computational modeling, designed mutations are introduced in the active site of the enzyme using the site-directed mutagenesis technology. After performing the targeted mutagenesis, the role of specific residues in covalent catalysis was verified through experiments.
- Enzyme Active Site Evaluation
Kinetic studies are performed on enzymes after site-directed mutagenesis to measure the rate of enzymatic reactions and to assess the effect of mutation on enzyme activity.
Applications
Our enzyme active site design strategy based on covalent catalysis can be used in a wide range of enzyme engineering applications.
- For Biomedical Field: Designing enzymes for processing in synthetic biology, modifying enzymes in metabolic pathways, and designing enzyme preparations for scientific research or disease diagnosis.
- For Food Manufacturing Field: Designing enzymes for raw material development to improve the quality of production processes in the dairy, brewing and fermented food industries.
- For Fine Chemicals Field: Designing and modifying enzyme additives for the detergent industry, modifying enzymes for raw material processing in the textile and paper industries, modifying industrial membrane preparations for the petrochemical industry.
CD Biosynsis strives to offer comprehensive and reliable enzyme active site design services for various applications, whether academic research, industrial applications, or biotechnological development, our services are tailored to meet your specific requirements. Our team is ready to assist you and provide the insights you need to achieve your goals. If you are interested in our services, please don't hesitate to contact us.
Reference
- Reed, CJ.; et al. Molecular understanding of heteronuclear active sites in heme-copper oxidases, nitric oxide reductases, and sulfite reductases through biomimetic modelling. Chem Soc Rev. 2021, 50(4):2486-2539.
