Enhancing Enzyme Stability through Evolutionary Trace

CD Biosynsis is a leading expert in the field of enzyme design and evolution, with a long and in-depth experience in enzyme directed evolution. Our dedicated team utilizes evolutionary trace analysis to extract key information from enzyme sequences, providing valuable insights for the rational design of enzymes with improved properties or novel functions.

Overview

Enzymes are biological macromolecules produced by living cells with special catalytic effects. However, most enzyme reactions in biological organisms need to be carried out under mild conditions, which also leads to poor stability and tolerance of enzymes. Also, the activity of enzymes is easy to be decreased in high heat, strong acidic environment or high ionic concentration, which greatly limits the in vitro application and promotion of enzymes. Therefore, the structural modification of enzymes to improve their stability or catalytic activity has been a hot and difficult issue in research.

With the wide application of the Evolutionary Trace (ET) analysis, the relationship between the amino acid sequence structure of enzymes and their function has been recognized. Through sequence comparison between homologous proteins and the relationship between sequence changes and evolutionary differences, it is possible to identify amino acid sites associated with thermal stability and to alter the molecular structure of enzymes through targeted mutagenesis or sequence design, thereby improving enzyme stability.

Fig 1. Critical arginine salt bridge mediating contact between the KS-AT linker. (Drufva EE, et al., 2020)

What We Can Offer

• Predict of Enzyme Modification Sites
  We can use the enzyme evolutionary trajectory approach, combined with computational tools such as homology modeling and molecular dynamics simulation, to predict regions in the enzyme that may be related to stability, thus guiding targeted efforts to improve enzyme stability.
• Providing Diversified Enzyme Structure Modification Methods
  Based on the prediction results of enzyme evolution tracking methods, we can provide corresponding enzyme structure modification strategies, bridging the gap between the prediction results of evolution tracking and the actual stability modification.

CD Biosynsis strives to offer comprehensive and reliable enzyme evolution trace analysis for various applications, whether academic research, industrial applications, or biotechnological development, our services are tailored to meet your specific requirements. Contact us today to learn more about our enzyme evolutionary trace analysis services and how they can benefit your research or development projects. Our team is ready to assist you and provide the insights you need to achieve your goals.

Reference

1. Drufva, EE.; et al. Site directed mutagenesis as a precision tool to enable synthetic biology with engineered modular polyketide synthases. Synth Syst Biotechnol. 2020, 5(2):62-80.