Fluorescence-Based High-Throughput Enzyme Assays

EnzymoGenius™ specializes in fluorescence-based high-throughput enzyme assays, providing cutting-edge solutions for researchers and scientists seeking robust enzymatic activity analysis. Our service integrates advanced technologies to deliver accurate, efficient, and high-throughput enzyme assays, supporting a diverse range of research topics.

Overview

Fluorescence-based high-throughput enzyme assays represent a pivotal technological advancement in bioanalytical research. These assays leverage the intrinsic fluorescence properties of certain molecules, often fluorophores, to monitor enzymatic activities rapidly and in a large-scale format. By exploiting the changes in fluorescence signals associated with enzymatic reactions, these assays offer a sensitive and efficient means to quantify enzyme kinetics and substrate turnover. Notably, recent research in this domain has focused on enhancing assay sensitivity, throughput, and versatility. Innovations include the development of novel fluorogenic substrates tailored to specific enzymes, as well as the integration of microfluidic platforms for streamlined analyses. Additionally, advancements in fluorescence detection technologies, such as time-resolved fluorescence and fluorescence polarization, have further refined the precision and applicability of high-throughput enzyme assays, positioning them as indispensable tools for studying diverse biological processes.

Fig. 1 Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7. (Kitakaze K, et al., 2021)

Leading Technology Advantages

• High Sensitivity Fluorescence Detection
  Our cutting-edge fluorescence detection technology ensures exceptional sensitivity, enabling the detection of subtle changes in enzymatic activity.
• Multiplexed Assays
  EnzymoGenius™ offers the capability to perform multiplexed assays, allowing simultaneous analysis of multiple enzymes or substrates in a single experiment, optimizing efficiency and resource utilization.
• Automation Integration
  Leveraging automated systems, our service enhances throughput while minimizing human error, guaranteeing reproducibility in experimental results.

EnzymoGenius™ extends its expertise to serve a broad spectrum of research areas, ranging from signal transduction pathways to drug discovery. Our unparalleled commitment to delivering precise, high-throughput enzyme assays ensures that researchers can unravel the intricacies of enzymatic processes with confidence. For inquiries or to discuss specific assay requirements, please contact us, and our team of experts will be delighted to assist you in advancing your research endeavors.

Reference

1. Kitakaze, K.; et al. Development of a selective fluorescence-based enzyme assay for glycerophosphodiesterase family members GDE4 and GDE7. JACS Au. 2021, 62: 100141.