Switching Substrate Specificity using Active Site Mutations

EnzymoGenius™ specializes in the innovative field of substrate specificity modification through active site mutations. Our cutting-edge service empowers researchers and industries to tailor enzymatic functions for diverse applications. Leveraging advanced technologies, we facilitate precise alterations to enzyme active sites, enabling the customization of substrate preferences to meet specific needs.

Overview

Switching substrate specificity through active site mutations is a strategic approach in enzymatic research aimed at modulating enzymatic function. This process involves targeted mutations within the enzyme's active site, altering its substrate binding preferences. Noteworthy progress in this field has been achieved through structural and computational analyses, providing insights into the molecular mechanisms governing substrate recognition. Researchers have systematically explored the effects of specific amino acid substitutions on substrate specificity, elucidating the intricate interplay between enzyme structure and function. This nuanced understanding facilitates the design of enzymes with tailored substrate preferences, holding promise for applications in biotechnology and enzyme engineering. Ongoing investigations continue to refine and expand our knowledge of this innovative approach, unlocking new possibilities for manipulating enzymatic activities in a controlled manner.

Fig. 1 Effect of altered mobility of the active site on the specificity of methionine adenosyltransferase. (Gade M, et al.,2021)

Leading Technologies

• Computational Modeling
  Harness the power of advanced computational tools to predict and analyze the impact of active site mutations on substrate binding.
• CRISPR-Cas9 Technology
  Utilize CRISPR-Cas9 gene editing for precise and efficient introduction of mutations into enzyme-encoding genes.
• High-Throughput Screening
  Implement high-throughput screening methods to rapidly evaluate large libraries of enzyme variants, expediting the optimization process.
• Structural Biology Techniques
  Employ cutting-edge structural biology techniques, such as X-ray crystallography and NMR spectroscopy, to elucidate the three-dimensional structures of engineered enzymes.
• Interdisciplinary Expertise
  Benefit from our team's interdisciplinary expertise, combining biology, bioinformatics, and bioengineering to deliver comprehensive solutions.

EnzymoGenius™ offers a transformative service to customize substrate specificity through active site mutations. Our comprehensive process, utilizing state-of-the-art technologies, ensures precise modifications, making enzymes adaptable to diverse research and industrial needs. From consultation to optimization, our interdisciplinary team brings together expertise in biology, bioinformatics, and bioengineering to deliver tailored solutions. Contact us to explore the possibilities and revolutionize enzymatic functions for your specific applications.

Reference

1. Gade, M.; et al. Substrate Dynamics Contribute to Enzymatic Specificity in Human and Bacterial Methionine Adenosyltransferases. JACS Au. 2021, 1(12): 2349-2360.