Strain Engineering for 3-Hydroxypropionic Acid

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CD Biosynsis is able to integrate genomic, proteomic, and metabolomic data using genetic, metabolic, and enzymatic engineering tools to develop and optimize microbial synthetic pathways for 3-hydroxypropionic acid. We offer customized synthetic biology services designed to help our customers achieve efficient biosynthesis of 3-hydroxypropionic acid.

Advantages of Synthetic Biology-Driven 3-Hydroxypropionic Acid Production

3-Hydroxypropionic acid is an important platform chemical raw material. 3-Hydroxypropionic acid can be used as an important chemical intermediate for the production of acrylic acid, acrylamide, malonic acid, 3-hydroxypropionic acid polymer, etc. Currently, 3-hydroxypropionic acid is produced by chemical synthesis. However, the chemical synthesis method has disadvantages such as high technical difficulty and environmental pollution. Synthetic biology is able to synthesize 3-hydroxypropionic acid using substrates such as glucose and glycerol, and this method has the advantages of simple operation, low production cost, and environmental friendliness.

Figure 1. Strategies for high-level production of 3-hydroxypropionic acid. (Zhao P, et al., 2021)

What We Provide

Optimization of Fermentation Conditions

We are able to help our customers optimize the method of 3-hydroxypropionic acid separation and purification, and simplify the pathway of 3-hydroxypropionic acid production. We help our customers minimize the by-products of 3-hydroxypropionic acid production by optimizing the fermentation conditions.

Genetic Engineering

We can use genetic engineering tools to increase the growth rate and tolerance to acidic environments of microbial cells in 3-hydroxypropionic acid synthesis medium.

Multi-omics Analysis

We are able to integrate genomics, proteomics, and metabolomics into the design, construction, and testing of 3-hydroxypropionic acid biosynthesis systems to improve the yield of 3-hydroxypropionic acid.

Deliverables

Efficient cell factory for 3-hydroxypropionic acid production.
3-hydroxypropionic acid.

How We Help

Currently, the biosynthesis of 3-hydroxypropionic acid mainly uses glucose and glycerol as substrates. Based on the synthetic biology platform, CD Biosynsis is able to provide optimization services for the natural synthetic pathway of 3-hydroxypropionic acid. If the following services do not meet your requirements, please contact us directly and we look forward to working with you.

Optimum the 3-Hydroxypropionic Acid Production Pathway from Propionic Acid

Our scientists work on the optimization of the natural synthetic pathway of 3-hydroxypropionic acid. We are able to help our customers screen for key enzymes that promote the production of 3-hydroxypropionic acid and improve key enzyme activity through gene editing techniques.

Optimum the 3-Hydroxypropionic Acid Production Pathway from Lactic Acid

Lactic acid and 3-hydroxypropionic acid are isomers. We can help our customers to optimize the separation and recovery methods in the production of 3-hydroxypropionic acid by using lactic acid as a substrate and to improve the efficiency of 3-hydroxypropionic acid production.

Optimum the 3-Hydroxypropionic Acid Production Pathway from β‑Alanine Pathway

We can help our customers overexpress key enzymes involved in the β-alanine pathway or design artificial enzymes that can increase the yield of 3-hydroxypropionic acid.

Optimum the 3-Hydroxypropionic Acid Production Pathway from Glucose

The shortest pathway for the synthesis of 3-hydroxypropionic acid using glucose as a substrate is the malonyl-CoA pathway. We are able to promote the synthesis of 3-hydroxypropionic acid by enhancing metabolic flux, enhancing cofactors, and inducing point mutations in the genes encoding.

Optimum the 3-Hydroxypropionic Acid Production Pathway from Glycerol

Glycerol is inexpensive and there are natural pathways in many microorganisms that rely on it to produce 3-hydroxypropionic acid. Based on our synthetic biology platform, we are able to help our customers to realize microbial chassis development and optimization services for the synthesis of 3-hydroxypropionic acid microsomes using glycerol as a substrate.

The following are microbial chassis that have been successfully or are under development for 3-hydroxypropionic acid biosynthesis.

Escherichia coli	Corynebacterium glutamicum	Aspergillus
Lactobacillus roxellanae	Hansenula miso	Fusarium
merismoides	Candida rugosa	Byssochlamys sp.
Klebsiella pneumoniae	Synechococcus elongatus

Applications of 3-Hydroxypropionic Acid

CD Biosynsis can develop tailored tools and customized approaches to harness the power of synthetic biology to drive 3-hydroxypropionic acid production and meet the needs of customers in a variety of industries.

Used as an intermediate for the production of 1,3-propanediol.
Used as a platform chemical for the production of acrylic acid.
Used as a raw material for bio-polyester.
Used as a nematocide.

Want to Learn More?

As a rapidly growing synthetic biology company, CD Biosynsis is committed to helping our customers meet the growing and evolving demand for bio-based chemical production. All of our deliverables will undergo a rigorous quality testing process to ensure the quality and reliability and can be delivered on time. If you are interested in our services or have any further questions, please do not hesitate to contact us.

Reference

Zhao P, et al. Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria. World J Microbiol Biotechnol. 2021 Jun 15; 37(7): 117.

Please note that all services are for research use only. Not intended for any clinical use.

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