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ALT/GPT Activity Assay Kit - Fluorometric

Catalog Number: AKAS0088

Price: Online Inquiry

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Specifications

Product Information
Product Name ALT/GPT Activity Assay Kit - Fluorometric
Instruments Microplate Reader
Sample Type Serum, Plasma, Animal Tissue
Research Area Diabetes & Obesity Enzyme Activity
Detection Principle ALT catalyze the amino conversion reaction between alanine and α-ketoglutaric acid to produce pyruvic acid and glutamic acid. Under the action of pyruvate oxidase, pyruvic acid generates H2O2, which reacts with the non-fluorescent substance to form fluorescent substance under the action of peroxidase. The activity of ALT can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
Assay Time 75 min
Storage -20°C
Shipping Conditions Gel Pack

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