Amino Acid Strain Engineering

Our service for amino acid biosynthesis has been meticulously developed to facilitate the precise manipulation and generation of amino acids using state-of-the-art synthetic biology methodologies. Harnessing the potential of genetic manipulation, CD Biosynsis empowers the sustainable and economically efficient production of amino acids, catering to a diverse array of applications in industries, pharmaceuticals, and scientific research.

Fig 1. The regulation of the sulfur amino acid biosynthetic pathway in Cryptococcus neoformans. (De Melo, et al., 2019)

Background

Amino acids are the fundamental building blocks of proteins and play a pivotal role in various biological processes. Amino acid biosynthesis is a complex and highly regulated set of biochemical pathways through which organisms synthesize these essential compounds. The advantages of synthesizing amino acids using synthetic biology methods include:

• Sustainability. Amino acid biosynthesis offers a sustainable alternative to traditional methods of amino acid production, such as extraction from natural sources or chemical synthesis.
• Purity and Quality. Through controlled fermentation processes, we can achieve high levels of purity and quality in the synthesized amino acids.
• Cost Efficiency. Over time, advances in biotechnology have made amino acid biosynthesis increasingly cost-effective. This translates to competitive pricing for our clients while maintaining product quality.

Amino Acid Biosynthesis Pathway

Amino acid biosynthesis involves a series of enzymatic reactions organized into specific pathways. These pathways are intricate and differ for each amino acid. However, some common principles apply:

Types of Amino Acids We Can Biosynthesize

Our synthetic biology technology enables us to biosynthesize a wide range of amino acids, including but not limited to:

• Essential Amino Acids. These are amino acids that the human body cannot synthesize independently and must be obtained through diet or supplementation. Examples include lysine, leucine, and valine.
• Non-Essential Amino Acids. These amino acids can be synthesized by the human body, but their availability through biosynthesis allows for applications in various industries. Examples include glutamine, glycine, and serine.
• Conditional Amino Acids. These amino acids are typically non-essential but become essential under certain conditions, such as illness or stress. Examples include arginine and tyrosine.
• Specialty Amino Acids. Some amino acids have specific applications in industries like pharmaceuticals, cosmetics, and food production. Examples include cysteine, tryptophan, and aspartic acid.

Our Technical Advantages

• Metabolic Engineering: We modify microorganisms genetically to create strains capable of amino acid production, ensuring efficiency and precision.
• Fermentation Expertise: Our bioreactors are equipped with advanced control systems to optimize microbial growth and amino acid production by regulating factors like temperature, pH, and nutrients.
• Quality Control: Rigorous quality control measures are in place to ensure that the synthesized amino acids meet or exceed industry standards. This includes analytical techniques such as high-performance liquid chromatography (HPLC) and mass spectrometry.

Whether you require custom-engineered microorganisms, bioprocess development, or amino acid screening, our team of experts is here to meet your unique needs. With our cutting-edge technology and commitment to excellence, we empower innovation across various sectors. Contact us today to explore how our biosynthesis capabilities can drive your success.

Reference

1. De, Melo.; et al. The regulation of the sulfur amino acid biosynthetic pathway in Cryptococcus neoformans: the relationship of Cys3, Calcineurin, and Gpp2 phosphatases. Scientific Reports. 2019, 9(1), 11923.

Please note that all services are for research use only. Not intended for any clinical use.