ANO1 Knockout cell line （PC-3）

The ANO1 Knockout cell line (PC-3) is primarily utilized in cancer research specifically in studies focusing on prostate cancer and ion channel physiology Researchers leverage this cell line to investigate the role of the ANO1 (TMEM16A) gene in tumorigenesis metastatic behavior and drug resistance A prominent use case is in drug screening assays where the absence of ANO1 is evaluated for altered cellular responses to chemotherapeutic agents enabling the identification of potential therapeutic targets

In experimental workflows the ANO1 Knockout cell line can be cultured under standard conditions and employed in functional assays such as calcium imaging and viability tests to assess cellular behavior in the absence of ANO1 signaling This can lead to a deeper understanding of calcium-activated chloride channels and their impacts on cell proliferation and migration

The practical benefits of using this cell line include reduced variability in experimental results and enhanced clarity in delineating the functional roles of ANO1 in prostate cancer biology Additionally techniques such as CRISPR gene editing and RNA interference can be implemented efficiently with this model making it a versatile tool in translational research