Bacterial Codon Optimization Service

Overview

Bacterial codon optimization services involve modifying the DNA sequence of a gene to enhance its expression in bacterial hosts. Codon optimization adjusts the codon usage to match the preferences of the bacterial expression system, ensuring efficient translation and high levels of protein production. This process considers factors such as codon bias, GC content, mRNA secondary structure, and regulatory elements. By optimizing the codon usage, researchers can significantly improve the yield and functionality of recombinant proteins expressed in bacteria.

To improve protein expression by codon optimization, strategies depend on three essential assumptions. First, rate of protein synthesis is limited by rare codons. Second, without affecting protein structure and function, synonymous codons can be used interchangeably. Third, optimization can be improved through increased production of protein by replacing rare codons with frequently used ones.

Advantages

• Increased Protein Yield: By matching codon usage to the host, translation efficiency is improved, leading to higher protein expression levels.
• Enhanced Protein Functionality: Optimized codons can improve protein folding and stability, resulting in more functional proteins.
• Reduced Expression Time: Faster protein synthesis due to improved translation efficiency, reducing the time required for protein production.
• Cost-Effective Production: Higher yields and faster expression times lead to cost savings in large-scale protein production.
• Custom Solutions: Tailored optimization for specific bacterial hosts and expression systems to meet unique research and production needs.