Biodegradation Pathway Engineering

Biodegradation Pathway Engineering in Synthetic Biology

Synthetic biology has emerged as a promising approach for engineering microorganisms to perform complex metabolic tasks, including biodegradation. Biodegradation pathway engineering involves the modification of microbial metabolism to degrade specific contaminants or pollutants. At CD Biosynsis, we offer a range of services for biodegradation pathway engineering, utilizing the latest synthetic biology tools and techniques to design and optimize microbial consortia for biodegradation applications.

Figure 1: Schematic diagram of the biodegradation of toxic recalcitrant compounds. (Kurumbang N.P., et al. 2014)

Our Services for Biodegradation Pathway Engineering

Strain Identification and Characterization

CD Biosynsis offers a range of services for strain identification and characterization for biodegradation pathway engineering:

• Microbial strain isolation and culture-dependent methods.
• Culture-independent techniques, including metagenomics and metatranscriptomics.
• Genomic and proteomic analysis for metabolic pathway characterization.
• Strain selection and optimization for specific biodegradation applications.

Our team of experts has extensive experience in microbial ecology, environmental microbiology, and genomics, allowing us to identify and isolate a diverse range of microbial strains from various samples. We use genomic and proteomic analysis to characterize the metabolic capabilities of the identified strains, providing valuable insights into their biodegradation potential.

Pathway Design and Optimization

• Computational modeling and simulation of metabolic pathways.
• Flux balance analysis for pathway optimization.
• High-throughput screening for pathway selection and optimization.
• CRISPR-Cas genome editing and metabolic engineering for pathway modification and optimization.

We use a variety of computational and experimental approaches to design and optimize biodegradation pathways, ensuring that the pathways are highly effective and efficient.

Strain Construction and Testing

• CRISPR-Cas genome editing for strain modification.
• Plasmid-based gene expression for metabolic pathway construction.
• Batch and continuous culture experiments for strain testing and optimization.
• Metabolic profiling and gene expression analysis for pathway performance evaluation.

Scale-Up and Commercialization

• Process optimization for large-scale biodegradation production.
• Environmental sustainability assessment and optimization.
• Consulting services for regulatory compliance, intellectual property protection, and technology transfer.

Our Advantages

• Technical Expertise
• Customized Solutions
• Cost-Effective and Environmentally Sustainable
• Comprehensive Services

CD Biosynsis offers a comprehensive range of services for biodegradation pathway engineering, utilizing the latest synthetic biology tools and techniques to design and optimize microbial consortia for biodegradation applications. Our team of experts has extensive experience in synthetic biology, metabolic engineering, and biodegradation pathway engineering, providing clients with the highest level of technical expertise and support. Contact us today to learn more about our services for biodegradation pathway engineering.

References

1. Kurumbang N.P., et al. Computer-Assisted Engineering of the Synthetic Pathway for Biodegradation of a Toxic Persistent Pollutant. ACS Synthetic Biology, 2014, 3(3): 172-181.
2. Nikel P.I., De L.V. Engineering an anaerobic metabolic regime in Pseudomonas putida KT2440 for the anoxic biodegradation of 1,3-dichloroprop-1-ene. Metabolic Engineering, 2013, 15: 98-112.

Please note that all services are for research use only. Not intended for any clinical use.