Creatinine Strain Engineering

CD Biosynsis is dedicated to providing top-tier technical services for the biosynthesis of creatinine, exclusively for scientific research purposes. With a commitment to excellence and a deep understanding of biotechnology, we offer comprehensive solutions that meet the exacting standards of the scientific community.

Background

Creatinine is a naturally occurring organic compound primarily found within the body as a metabolic byproduct of creatine phosphate. It plays a pivotal role in energy metabolism, primarily in skeletal muscles. Creatinine is a nitrogenous waste product and is continuously generated in the body as a result of muscle contraction. It is excreted primarily through the kidneys, making it a valuable biomarker for assessing renal function.

Fig 1. Schematic of de novo creatine synthesis. (Muccini A M, et al., 2021)

The biosynthesis of creatinine involves intricate biochemical pathways within living organisms. Creatinine is synthesized primarily in the liver, where arginine and glycine serve as the precursor molecules. The enzymatic conversion of arginine to ornithine, followed by the transformation of ornithine to citrulline and argininosuccinate, ultimately results in the formation of arginine and its subsequent conversion into creatinine. This complex process underscores the importance of using biosynthesis to ensure accuracy and precision in research applications.

Creatinine Biosynthesis Pathway

The biosynthesis of creatinine involves a series of intricate biochemical steps:

How We Can Help

Leveraging advanced techniques in bioprocess engineering and enzymology, we offer a range of services to enhance creatinine production and facilitate research in this area. Our technology includes:

• Enzyme Engineering. We employ state-of-the-art enzyme engineering techniques to enhance the activity and specificity of enzymes involved in the creatine biosynthesis pathway.
• Metabolic Development. Our team utilizes metabolic engineering strategies to enhance the efficiency of creatine synthesis within host organisms, such as bacteria or yeast.
• Bioprocess Optimization. We offer expertise in optimizing bioprocess conditions, including fermentation, to maximize creatinine yield.
• Analytical Services. We provide comprehensive analytical services to quantify creatinine levels accurately in various matrices, such as blood and urine.
• Customized Solutions. We collaborate closely with our clients to develop innovative approaches for creatinine biosynthesis, addressing unique challenges and objectives.

CD Biosynsis is committed to delivering the highest quality services that empower researchers to advance their work with precision and reliability. If you seek to harness the potential of biosynthesized creatinine for your research endeavors, do not hesitate to contact us. Our team of experts is ready to assist you in achieving your scientific goals. Contact us today to explore the possibilities.

Reference

1. Muccini A. M.; et al. Creatine Metabolism in Female Reproduction, Pregnancy and Newborn Health. Nutrients. 2021, 13(2):490.

Please note that all services are for research use only. Not intended for any clinical use.