Insulin Strain Engineering

Our specialized biotechnology services are dedicated to the precise biosynthesis of insulin, exclusively for scientific research purposes. Our commitment is to provide state-of-the-art technical solutions in the realm of insulin production, catering to the exacting needs of researchers and laboratories.

Background

Insulin is a pivotal hormone in regulating glucose metabolism within the human body. It plays a fundamental role in maintaining blood glucose homeostasis, allowing cells to absorb glucose from the bloodstream, which is essential for cellular energy production. Discovered in the early 20th century, insulin has since been a life-saving therapy for individuals suffering from diabetes mellitus, a condition characterized by either insufficient insulin production or impaired insulin action.

Fig 1. Schematic of insulin production and secretion. (Vasiljević J, et al., 2020)

The biosynthesis of insulin is a complex and highly regulated process that occurs in the beta cells of the pancreas. This intricate process involves the transcription and translation of the insulin gene, followed by post-translational modifications to convert proinsulin into the biologically active form of insulin. Producing insulin via biosynthesis has become a critical endeavor in the field of biotechnology, given its importance in treating diabetes.

Insulin Biosynthesis Pathway

The biosynthesis of insulin can be divided into several distinct stages, each involving specific molecular events and cellular processes:

Transcription: Transcription factors, such as PDX1 and NeuroD1, bind to the insulin gene promoter, initiating transcription. Preproinsulin mRNA is synthesized in the nucleus and then transported to the endoplasmic reticulum (ER).

Translation: Preproinsulin is synthesized as a single-chain precursor with a signal peptide.The signal peptide directs the nascent preproinsulin to the ER, where it is translocated into the ER lumen.

Post-Translational Modifications: In the ER, the signal peptide is cleaved, yielding proinsulin.Proinsulin undergoes proper folding and disulfide bond formation and is transported to the Golgi apparatus for further modification.

Proteolytic Cleavage: Within the Golgi apparatus, proinsulin is cleaved by specific enzymes into insulin and C-peptide.The resulting insulin molecules are stored in secretory vesicles within the beta cells.

Release and Regulation: Upon stimulation, such as a rise in blood glucose levels, insulin-containing vesicles fuse with the cell membrane.Insulin is released into the bloodstream, allowing it to exert its glucose-lowering effects on target tissues.

How We Can Help

We offer a range of cutting-edge technologies and services to optimize the insulin biosynthesis process:

• Genetic Engineering and Recombinant DNA Technology

We employ advanced genetic engineering techniques, including customized plasmid design, gene insertion, and gene expression optimization, to design and engineer host cells capable of producing insulin efficiently.

• Fermentation and Cell Culture

Our state-of-the-art bioreactors and cell culture expertise enable large-scale production of insulin-producing cells. We optimize culture conditions, media formulations, and process parameters to enhance insulin yield.

• Continuous Innovation

Continuously seeking ways to optimize insulin biosynthesis processes and improve product quality.

• Advanced Purification Methods

We utilize advanced purification methods, including chromatography and filtration, to isolate and purify insulin to pharmaceutical-grade standards.

CD Biosynsis invites you to connect with us, ensuring access to the highest quality biosynthesized insulin, impeccable technical expertise, and unwavering commitment to the success of your scientific pursuits. Contact us today to explore how our services can elevate your research to new heights.

Reference

1. Vasiljević, J.; et al. The making of insulin in health and disease. Diabetologia. 2020, 63, 1981–1989.

Please note that all services are for research use only. Not intended for any clinical use.