Cas9 Expressing Human retinal pigment epithelial cells

ARPE-19-CAS9 is an innovative and highly specialized human retinal pigment epithelial cell line engineered to stably express the CRISPR-associated protein 9 (Cas9) nuclease Derived from the human retina this cell line mimics native retinal epithelial properties providing an essential tool for genetic manipulation and functional studies pertinent to ocular biology and related diseases

The ARPE-19-CAS9 cells are maintained in a culture medium consisting of a carefully formulated mixture that promotes optimal growth enabling researchers to achieve a robust proliferative behavior akin to native retinal cells The stable integration of the Cas9 gene is accomplished using a specialized lentiviral vector allowing for reliable and efficient genome editing capabilities This ensures researchers can effectively leverage the CRISPR/Cas9 system to introduce targeted modifications in the genome facilitating investigations into gene function disease mechanisms and therapeutic interventions specifically related to vision disorders

In terms of applications ARPE-19-CAS9 serves as a critical platform for studying retinal degenerative diseases such as age-related macular degeneration and retinitis pigmentosa By manipulating genes implicated in these conditions researchers can construct models that mimic disease phenotypes providing insights into progressive mechanisms and identifying potential therapeutic targets Moreover the ability to rapidly edit the genome with high precision allows for the evaluation of gene function in a physiologically relevant context enhancing translational research focused on developing gene therapies

ARPE-19-CAS9 cells exhibit a hygromycin resistance marker integrated with the Cas9 expression ensuring selective maintenance of the Cas9-positive cell population throughout multiple passages Rigorous mycoplasma testing ensures that these cells are free from contamination upholding the experimental integrity critical for credible scientific outcomes Each vial of ARPE-19-CAS9 is provided at a cell density of 1×10^6 and is stored in liquid nitrogen with proper shipping protocols to maintain cell viability intact

As genomic medicine continues to evolve the ARPE-19-CAS9 cell line stands out as an invaluable resource for researchers aiming to enhance our understanding of ocular genetics and develop novel therapies for visual impairments Its utility spans from basic research to translational applications underscoring its significance in the biotechnology landscape