CD Acetate Assay Kit

Catalog Number: AKBA003

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$1347

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Specifications

Product Information
Product Name	CD Acetate Assay Kit
Applications	For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism.
Features	Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays. Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature, and read. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Serum, plasma, food, agriculture, and environment
Species	All
Assay Time	30 min
Limit of Detection	0.13 mM
Expiration Date	6 months
Description	Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar and is extensively used in food, dyes, paints, glue, and synthetic fibers. CD assay uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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