CD Acetoacetate Assay Kit

Catalog Number: AKBA059

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$1180

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Specifications

Product Information
Product Name	CD Acetoacetate Assay Kit
Applications	Direct assay of acetoacetate in serum, plasma, urine and other biological samples.
Features	Sensitive and accurate. Linear detection range of 0.012 to 1 mM (1.2 – 100 nmole/well) for acetoacetate in 96-well plate assay. Convenient. No sample pretreatment needed. The procedure involves adding a single working reagent and reading the optical density at room temperature in kinetic mode. High-throughput. Can be automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	OD550nm
Sample Type	Serum, plasma, urine, and other biological samples
Species	All
Assay Time	30 min
Limit of Detection	12 μM
Expiration Date	6 months
Description	ACETOACETATE is one of three ketone bodies produced in the liver mainly from oxidation of fatty acids and are normally present at low concentrations in urine and blood. Increased ketone concentrations in the blood may lead to metabolic acidosis, which has been associated with diabetes, childhood hypoglycemia, growth hormone deficiency, alcohol or salicylate intoxication and inborn errors of metabolism. Acetoacetate is an unstable ketone body that spontaneously decarboxylates to release carbon dioxide and acetone.Simple, direct and automation-ready procedures for measuring acetoacetate (AAT) are very desirable. BioAssay Systems’ Acetoacetate assay is based on a reaction with Sodium Nitroprusside forming a product with absorbance at 550 nm.
Storage	Storage: -20°C upon receipt.

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