CD Acetylcholine Assay Kit

Catalog Number: AKBA004

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$1377

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Specifications

Product Information
Product Name	CD Acetylcholine Assay Kit
Applications	For quantitative determination of acetylcholine and evaluation of drug effects on acetylcholine metabolism.
Features	Use 20 µL samples. Linear detection range: colorimetric assay 10 to 200 µM, fluorimetric assay 0.4 to 10 µM acetylcholine.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Serum, plasma, urine, saliva, milk, tissue, cell culture, etc
Species	All
Assay Time	30 min
Limit of Detection	OD, FL: 10, 0.4 µM
Expiration Date	6 months
Description	ACETYLCHOLINE is a neurotransmitter produced in acetylcholinergic neurons. It plays important roles in skeletal muscle movement, regulation of smooth and cardiac muscles, as well as in learning, memory, and mood. CD method provides a simple, direct, and high-throughput assay for measuring acetylcholine in biological samples. In this assay, acetylcholine is hydrolyzed by acetylcholinesterase to choline which is oxidized by choline oxidase to betaine and H2O2. The resulting H2O2 reacts with a specific dye to form a pink-colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the acetylcholine concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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