CD Acid Phosphatase Assay Kit

Catalog Number: AKBA061

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$537

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Specifications

Product Information
Product Name	CD Acid Phosphatase Assay Kit
Applications	For quantitative determination of acid phosphatase activity in biological samples.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.05 to 60 U/L for a 30-minute reaction. High-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD405nm
Sample Type	Biological (e.g. serum) samples
Species	All
Assay Time	30 min
Limit of Detection	0.05 U/L
Expiration Date	6 months
Description	Acid Phosphatase (ACP) is an enzyme that catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney, and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.BioAssay System’s non-radioactive, colorimetric ACP assay is based on the cleavage of p-nitrophenol from the synthetic substrate. p-Nitrophenol becomes intensely colored after the addition of the stop reagent. The increase in absorbance at 405 nm after the addition of the stop reagent is directly proportional to the enzyme activity.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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