CD Aconitase Assay Kit

Catalog Number: AKBA115

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$1197

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Specifications

Product Information
Product Name	CD Aconitase Assay Kit
Applications	For quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Species	All
Assay Time	30 min
Limit of Detection	0.5 U/L
Expiration Date	6 months
Description	ACONITASE (ACONITATE HYDRATASE) is an enzyme in the citric acid (TCA) cycle that catalyzes the conversion of citrate to isocitrate. The activity of aconitase depends largely upon the iron-sulfur [Fe4S4] 2+ cluster. Related diseases include aconitase deficiency (e.g. myopathy and exercise intolerance), Friedreich’s ataxia, and diabetes. BioAssay Systems’ aconitase assay measures the isocitrate generated as a product of the aconitase reaction. The isocitrate is then oxidized producing NADPH and the oxidation product. The NADPH converts the dye to an intense violet color with an absorption maximum of 565 nm. The increase in absorbance at 565 nm is directly proportional to aconitase activity.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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