CD Adipolysis Assay Kit

Catalog Number: AKBA117

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$1227

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Specifications

Product Information
Product Name	CD Adipolysis Assay Kit
Applications	For quantitative assay of adipolysis and evaluation of drug effects on adipolysis.
Features	Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.92 to 100 µg/mL (10 to 1000 µM) glycerol for colorimetric assays and 0.2 to 5 µg/mL for fluorimetric assays. Rapid and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature. Robust and amenable to HTS assays. Potential interference by testing drugs is greatly reduced at 570nm. Compatible with culture media containing phenol red. Assays can be performed in 96 or 384-well plates.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Cell culture media
Species	All
Assay Time	20 min
Limit of Detection	0.92 µg/mL
Expiration Date	12 months
Description	Obesity is a chronic condition that develops from the storage of excess energy in the form of adipose tissue. The resulting adiposity presents a high-risk factor for diseases such as type 2 diabetes, cardiovascular diseases, and cancer. ADIPOLYSIS or lipolysis is a highly regulated process in fat metabolism, in which triglycerides are broken down into glycerol and free fatty acids. Rapid, robust and accurate procedures for adipolysis quantification in cell culture are very useful in research and drug discovery. CD adipolysis assay kit directly measures glycerol released during adipolysis. This homogeneous assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm is directly proportional to glycerol concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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