CD ADP Assay Kit

Catalog Number: AKBA310

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$1317

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Specifications

Product Information
Product Name	CD ADP Assay Kit
Applications	For quantitative determination of ADP determination in cells and other biological samples.
Features	Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.1 µM ADP can be quantified. Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	FL530/590nm
Sample Type	Cells and other biological samples
Species	All
Assay Time	30 min
Limit of Detection	0.1 µM
Expiration Date	6 months
Description	Adenosine diphosphate (ADP) is the product of ATP dephosphorylation by ATPases. ADP can be converted back to ATP by ATP synthases. ADP levels regulate several enzymes involved in intermediary metabolism. Conventionally, ADP levels are measured by luciferase/luciferin-mediated assays after ADP is converted to ATP. However, since these assays require measurement of ATP in the sample before conversion of ADP to ATP, if the nascent ATP concentration is significantly higher than the ADP concentration, the ATP signal will drown out the ADP signal. BioAssay Systems’ newly designed ADP Assay Kit provides a convenient fluorometric means to measure ADP levels even in the presence of ATP. In the assay, ADP is converted to ATP and pyruvate. The generated pyruvate is then quantified by a fluorimetric method (lex/em = 530/590nm). The assay is simple, sensitive, stable, high-throughput adaptable, and can detect as low as 0.1 µM ADP in biological samples.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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