Product Information | |
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Product Name | CD AF Cholesterol Assay Kit |
Applications | For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism. |
Features | Sensitive and accurate. Linear detection range in 96-well plate: 0.1 to 10 mg/dL cholesterol for colorimetric assays and 0.02 to 2 mg/dL for fluorimetric assays. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. |
Detection Method | OD570nm, or FL530/585nm |
Sample Type | Serum, plasma, etc |
Species | All |
Assay Time | 40 min |
Limit of Detection | OD, FL: 1, 0.2 mg/dL |
Expiration Date | 12 months |
Description | CHOLESTEROL is a sterol and lipid present in the cell membranes and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer, and cerebral hemorrhage. Simple, direct, and automation-ready procedures for measuring cholesterol are very desirable. CD Cholesterol Assay uses a single Working Reagent that combines cholesterol ester hydrolysis, oxidation, and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the total cholesterol concentration in the sample. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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