CD Alcohol Dehydrogenase Assay Kit

Catalog Number: AKBA062

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$1227

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Specifications

Product Information
Product Name	CD Alcohol Dehydrogenase Assay Kit
Applications	For quantitative determination of alcohol dehydrogenase activity and evaluation of drug effects on its metabolism.
Features	Fast and sensitive. Linear detection range (20 µL sample): 0.4 to 80 U/L for 30 min reaction. Detection Limit of 0.1 U/L for 120 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological (e.g. plasma, serum, urine, tissue, and culture media)
Species	All
Assay Time	30 min
Limit of Detection	0.4 U/L
Expiration Date	6 months
Description	ALCOHOL DEHYDROGENASE (ADH) is an oxidoreductase that catalyzes the interconversion of alcohols and aldehydes or ketones. ADH is important in humans and other organisms for the breakdown of alcohols which may otherwise be toxic. In yeast and some bacteria, ADHs catalyze the opposite reaction and produce alcohol as part of fermentation. BioAssay System’s non-radioactive, colorimetric ADH assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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