CD Aldehyde Dehydrogenase Inhibitor Screening Kit

Catalog Number: AKBA282

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$984

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Product Information
Product Name	CD Aldehyde Dehydrogenase Inhibitor Screening Kit
Applications	For evaluation and high-throughput screen (HTS) of aldehyde dehydrogenase modulators.
Features	Rapid and reliable. Can be completed in under an hour. Homogenous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Compounds that affect aldehyde dehydrogenase activity
Species	All
Assay Time	30 min
Expiration Date	6 months
Description	ALDEHYDE DEHYDROGENASES (ALDHs) are a superfamily of oxidoreductases that catalyze the conversion of aldehydes to carboxylic acids. ALDH is crucial in the metabolism of alcohol as alcohol dehydrogenase breaks down ethanol to acetaldehyde. Acetaldehyde, which is toxic to the body, is in turn broken down by ALDH to acetic acid. Imbalances of aldehyde dehydrogenase have been linked to both alcoholism and alcohol sensitivity in people. Inhibitors of the enzyme have been used in cases to treat alcoholism in patients. Cancer stem cell populations also display a heightened activity of ALDH, making ALDH inhibition a promising anti-cancer therapy approach. CD Aldehyde Dehydrogenase Inhibitor Screening Kit is based on the enzymatic conversion of acetaldehyde to acetic acid and NADH by ALDH. The formed NADH in turn reduces a formazan reagent into a colored product the absorbance of which, measured at 565 nm, is proportional to the enzyme activity in the reaction. The percent inhibition of a test compound can be determined by comparing the activity of ALDH treated with a test compound to the activity of untreated ALDH.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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