CD Ammonia/Ammonium Assay Kit

Catalog Number: AKBA009

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$1137

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Specifications

Product Information
Product Name	CD Ammonia/Ammonium Assay Kit
Applications	For quantitative determination of ammonia/ammonium and evaluation of drug effects on their metabolism.
Features	High sensitivity and wide linear range. Use a 20 µL sample. Linear detection range from 24 to 1000 µM ammonia. Homogeneous and simple procedure. A simple “mix-and-measure” procedure allows reliable quantitation of ammonia within 30 minutes.
Detection Method	OD340nm, or FL360/450nm
Sample Type	Serum, plasma, urine, saliva, cell culture etc
Species	All
Assay Time	30 min
Limit of Detection	24 µM
Expiration Date	6 months
Description	Ammonia (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. It is synthesized through amino acid metabolism and is toxic when present at high concentrations. In the liver, ammonia is converted to urea through the urea cycle. Elevated levels of ammonia in the blood (hyperammonemia) have been found in liver dysfunction (cirrhosis), while hypoammonemia has been associated with defects in the urea cycle enzymes (e.g. ornithine transcarbamylase). Simple, direct, and automation-ready procedures for measuring NH3 are popular in research and drug discovery. CD ammonia assay is designed to directly measure NH3 and NH4+. In this assay, NADH is converted to NAD+ in the presence of NH3, ketoglutarate, and glutamate dehydrogenase. The decrease in optical density at 340 nm or fluorescence intensity at λex/em = 360/450 nm is directly proportionate to the NH3 concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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