CD α-Amylase Assay Kit

Catalog Number: AKBA053

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$1287

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Specifications

Product Information
Product Name	CD α-Amylase Assay Kit
Applications	For quantitative determination of α-amylase activity in biological samples
Features	Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 0.2 – 100 U/L α-amylase in a 96-well plate assay. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated to assay thousands of samples per day.
Detection Method	OD405nm
Sample Type	Biological samples
Species	All
Assay Time	20 min
Limit of Detection	0.2 U/L
Expiration Date	6 months
Description	α-AMYLASE (EC 3.2.1.1) belongs to the family of glycoside hydrolase enzymes that break down starch into glucose molecules by acting on α-1,4-glycosidic bonds. The α-amylases cleave at random locations on the starch chain, ultimately yielding maltotriose and maltose, as well as glucose and “limit dextrin” from amylose and amylopectin. In mammals, α-amylase is a major digestive enzyme. Increased α-amylase levels in humans are associated with salivary trauma, mumps due to inflammation of the salivary glands, pancreatitis and renal failure. Simple, direct and automation-ready procedures for measuring amylase activity are very desirable. BioAssay Systems’ α-Amylase Assay Kit provides a convenient colorimetric method to measure α-amylase activity in biological samples. In this assay, α-amylase hydrolyzes a synthetic substrate to release 2-chloro-4-nitrophenol. The rate of formation of the 2-chloro-4-nitrophenol, measured at 405 nm, is directly proportional to the enzyme activity.
Storage	Storage: -20°C upon receipt.

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