CD Ascorbic Acid Assay Kit

Catalog Number: AKBA010

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$1317

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Specifications

Product Information
Product Name	CD Ascorbic Acid Assay Kit
Applications	For quantitative determination of ascorbate (ascorbic acid) and evaluation of drug effects on ascorbic acid metabolism.
Features	Use 20 µL samples. Linear detection range: colorimetric assay 6 to 1000 µM, fluorimetric assay 1 to 100 µM ascorbic acid.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Serum, plasma, urine, saliva, milk, tissue, and cell culture
Species	All
Assay Time	10 min
Limit of Detection	OD, FL: 6, 1 µM
Expiration Date	6 months
Description	Ascorbic acid (the L-enantiomer commonly known as vitamin C) is an important antioxidant found in living organisms and applied as additives in food and other industrial processes. By reacting with reactive oxygen species, it protects the cell from oxidative damages. CD method provides a simple, direct and high-throughput assay for measuring ascorbic acid. In this assay, ascorbic acid is oxidized by ascorbate oxidase resulting in the production of H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the ascorbic acid concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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