CD Aspartate Transaminase Assay Kit

Catalog Number: AKBA012

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$1227

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Specifications

Product Information
Product Name	CD Aspartate Transaminase Assay Kit
Applications	For quantitative determination of aspartate transaminase (AST) activity and evaluation of drug effects on AST activity.
Features	Sensitive. Linear detection range: 2-100 U/L. Simple and convenient. This simple, convenient assay can be carried out in a microplate or a cuvette and takes only 10 min.
Detection Method	OD340nm
Sample Type	Serum, plasma etc
Species	All
Assay Time	10 min
Limit of Detection	2 U/L
Expiration Date	6 months
Description	Aspartate Transaminase (AST), also known as serum glutamic oxaloacetic transaminase (GOT) or aspartate aminotransferase (ASAT/AAT), facilitates the conversion of aspartate and a-ketoglutarate to oxaloacetate and glutamate. There are two isoenzymes in humans: GOT1 is a cytosolic isoenzyme derived from red blood cells and the heart; GOT2 is the mitochondrial isoenzyme found mainly in the liver. AST is elevated in liver and muscle diseases. It is part of diagnostic tests for liver function, myocardial infarction, acute pancreatitis, acute hemolytic anaemia, severe burns, acute renal disease and trauma. Simple, direct and automation-ready procedures for measuring AST activity find wide applications in research and drug discovery. CD AST activity assay is based on the quantification of oxaloacetate produced by AST. In this assay, oxaloacetate and NADH are converted to malate and NAD by the enzyme malate dehydrogenase. The decrease in NADH absorbance at 340 nm is proportionate to AST activity.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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