CD Bile Acid Assay Kit

Catalog Number: AKBA049

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$1018

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Specifications

Product Information
Product Name	CD Bile Acid Assay Kit
Applications	For quantitative determination of total bile acids and evaluation of drug effects on bile acid metabolism.
Features	Safe. Non-radioactive assay. Sensitive and accurate. Linear detection range of 1 – 150 µM bile acids. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	FL530/585nm
Sample Type	Serum, plasma, urine, and other biological samples
Species	All
Assay Time	20 min
Limit of Detection	1 µM
Expiration Date	6 months
Description	BILE ACIDS. Twelve different types of bile acids are typically found in mammals, among them two primary types are cholic acid and chenodeoxycholic acid. These can be dehydroxylated into secondary bile acids. Finally, these four can be conjugated to either taurine or glycine creating 8 different conjugated bile acids. Bile acid levels in feces, blood, urine, and bile can be used as markers for various diseases such as hyperlipidemia, cholestasis, gallstones, colon cancer, etc. Bile acids also exist in a sulfate salt form known as bile acid-sulfates. Sulfation of bile acids increases their solubility and decreases intestinal absorption, thereby enhancing fecal and urinary excretion. This assay does not measure bile acid-sulfates, and measures only the twelve non-sulfated bile acids. BioAssay Systems’ Bile Acid Assay Kit provides a convenient fluorimetric means to measure total bile acids in biological samples. In the assay, 3α-hydroxysteroid dehydrogenase reacts with all twelve bile acids, converting NAD to NADH, which reduces a probe to a highly fluorescent product. The resulting fluorescence intensity (λex/em = 530/585 nm) is linear to the bile acid concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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