CD Calcium Assay Kit

Catalog Number: AKBA067

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$1197

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Specifications

Product Information
Product Name	CD Calcium Assay Kit
Applications	For quantitative determination of calcium ion Ca2+ and evaluation of drug effects on calcium metabolism.
Features	Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.08 mg/dL (20 µM) to 20 mg/dL (5 mM) Ca2+ in a 96-well plate assay. Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 3 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipids, protein,s, and minerals such as magnesium, iron, and zinc.
Detection Method	OD612nm
Sample Type	Biological, food, and environment
Species	All
Assay Time	3 min
Limit of Detection	0.08 mg/dL (20 µM)
Expiration Date	12 months
Description	CALCIUM is measured to monitor diseases of bone or calcium regulation disorders. Increased calcium levels in serum are reported in hyperparathyroidism, metastatic bone lesions, and hypervitaminosis, while decreased levels are observed in hypoparathyroidism, nephrosis, rickets, steatorrhea, nephritis, and calcium-losing syndromes. Urinary calcium levels aid the clinician in understanding how the kidneys handle calcium in certain diseases of the parathyroid gland. Urinary calcium levels are also essential in the medical evaluation of kidney stones. Simple, direct, and automation-ready procedures for measuring calcium concentration in biological samples are becoming popular in Research and Drug Discovery. CD calcium assay kit is designed to measure calcium directly in biological samples without any pretreatment. A phenol sulphone phthalein dye in the kit forms a very stable blue-colored complex specifically with free calcium. The intensity of the color, measured at 612 nm, is directly proportional to the calcium concentration in the sample. The optimized formulation minimizes any interference by substances such as magnesium, lipid, protein, and bilirubin.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

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