CD Cell Viability Assay Kit

Catalog Number: AKBA256

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$657

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Specifications

Product Information
Product Name	CD Cell Viability Assay Kit
Applications	Homogeneous assay for cell viability, proliferation, cytotoxicity and high-throughput screen for anticancer agents.
Features	Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay). Sensitive and accurate. As low as 100 cells can be accurately quantified. Time efficient. High-throughput assay in 96-well and 384-well plates allows the simultaneous processing of tens of thousands of samples per day. Homogeneous and convenient. A single reagent and “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS. Z factors of 0.6 to 0.9 are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.
Detection Method	FL530/590nm
Sample Type	Cell culture
Species	All
Assay Time	Assay takes 1-5 hrs, hands-on time 30 min
Limit of Detection	100 cells
Expiration Date	12 months
Description	This homogeneous assay involves simply adding a single reagent, the reagent, to the cell culture and measuring the fluorescence intensity (excitation wavelength = 530 – 570 nm, emission wavelength = 590 – 620 nm) after an incubation step. The reagent, like other resazurin-based assays such as the Alamar Blue reagent, utilizes the redox dye resazurin which is not fluorescent, but upon reduction by metabolically active cells is converted into a highly fluorescent product (resorufin). Living cells can readily reduce this non-toxic reagent and the resulting increase in fluorescence intensity can be conveniently monitored using a fluorescence spectrophotometer or plate reader. Nonviable cells have no metabolic capacity and, thus, will not reduce the dye. Therefore, the fluorescence intensity observed in this assay is a true measure of the viable cells. The reagent has been optimized for maximum sensitivity, reproducibility and long shelf-life. The homogeneous cell-based assay can be performed in multi-well plates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates. Applications include cell proliferation, cytotoxicity and apoptosis.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

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