CD Chloride Assay Kit

Catalog Number: AKBA068

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$1047

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Specifications

Product Information
Product Name	CD Chloride Assay Kit
Applications	For quantitative determination of chloride ion and evaluation of drug effects on chloride metabolism.
Features	Sensitive and accurate. Use as little as 5 µL samples. Linear detection range 0.7 mg/dL (0.2 mM) to 35 mg/dL (10 mM) Cl– in 96-well plate assay. Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 5 min. Can be readily automated as a high-throughput assay for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced the reagent and signal stability. Cuvet or 96-well plate assay. Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on raw biological samples i.e., in the presence of lipids, proteins, and minerals such as magnesium, iron, and zinc.
Detection Method	OD610nm
Sample Type	Biological, food, and environment
Species	All
Assay Time	5 min
Limit of Detection	0.7 mg/dL (0.2 mM)
Expiration Date	12 months
Description	Chloride is the major extracellular anion in human body fluids. Chloride plays a key role in maintaining proper water distribution, osmotic pressure, and electrolyte balance. Low chloride concentrations may be found with prolonged vomiting, extensive burns, metabolic acidosis, Addisonia crisis, and renal diseases. Elevated chloride concentrations are associated with dehydration, congestive heart failure, hyperventilation and urinary obstructions. Determination of chloride in sweat is useful in diagnosing cystic fibrosis. Simple, direct, and automation-ready procedures for measuring chloride concentration in biological samples are becoming popular in Research and Drug Discovery. CD chloride assay kit is designed to measure chloride directly in biological samples without any pretreatment. The improved Fried method utilizes mercuric 2,4,6-tripyridyl-s-triazine, which forms a colored complex specifically with chloride. The intensity of the color, measured at 610nm, is directly proportional to the chloride concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Shipping Conditions	Shipping: RT
Storage	Storage: 4°C upon receipt

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