CD Cholesterol Assay Kit

Catalog Number: AKBA013

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$1347

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Specifications

Product Information
Product Name	CD Cholesterol Assay Kit
Applications	For quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism.
Features	Sensitive and accurate. The detection limit of 5 mg/dL, linearity up to 300 mg/dL cholesterol in a 96-well plate assay. Convenient. Room temperature assay. No 37°C heater is needed. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	OD340nm
Sample Type	Serum, plasma etc
Species	All
Assay Time	40 min
Limit of Detection	5 mg/dL
Expiration Date	6 months
Description	CHOLESTEROL is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral haemorrhage. Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. CD Cholesterol Assay is based on cholesterol esterase hydrolysis of cholesterol esters to form free cholesterol and cholesterol dehydrogenase catalyzed conversion of cholesterol to cholest-4-ene-3-one, in which NAD is reduced to NADH. The optical density of the formed NADH at 340 nm is directly proportionate to the cholesterol concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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