Product Information | |
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Product Name | CD Choline Assay Kit |
Applications | For quantitative determination of choline and evaluation of drug effects on choline metabolism. |
Features | Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline. |
Detection Method | OD570nm, or FL530/585nm |
Sample Type | Serum, plasma, urine, saliva, milk, tissue, cells etc |
Species | All |
Assay Time | 30 min |
Limit of Detection | OD, FL: 1, 0.2 µM |
Expiration Date | 6 months |
Description | CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illnesses such as anxiety. CD method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink-colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample. |
Shipping Conditions | Shipping: On Ice |
Storage | Storage: -20°C upon receipt |
Please note that all services are for research use only. Not intended for any clinical use.
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