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(AKBA014)CD Choline Assay Kit

CD Choline Assay Kit

Catalog Number: AKBA014

Price:
$1347
Specifications Downloads Related products

Specifications

Product Information
Product Name CD Choline Assay Kit
Applications For quantitative determination of choline and evaluation of drug effects on choline metabolism.
Features Use 20 µL samples. Linear detection range: colorimetric assay 1 to 100 µM, fluorimetric assay 0.2 to 10 µM choline.
Detection Method OD570nm, or FL530/585nm
Sample Type Serum, plasma, urine, saliva, milk, tissue, cells etc
Species All
Assay Time 30 min
Limit of Detection OD, FL: 1, 0.2 µM
Expiration Date 6 months
Description CHOLINE and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illnesses such as anxiety. CD method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to βine and H2O2 which reacts with a specific dye to form a pink-colored product. The color intensity at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the choline concentration in the sample.
Shipping Conditions Shipping: On Ice
Storage Storage: -20°C upon receipt

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