CD D-Lactate Assay Kit

Catalog Number: AKBA050

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$1377

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Specifications

Product Information
Product Name	CD D-Lactate Assay Kit
Applications	For quantitative determination of D-lactate (D-lactic acid) and evaluation of drug effects on its metabolism.
Features	Sensitive and accurate. Detection limit of 1 µM and linearity up to 50 µM D-lactate in 96-well plate assay. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay. High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	FL530/585 nm
Sample Type	Serum, plasma, cell culture media etc
Species	All
Assay Time	60 min
Limit of Detection	1 µM
Expiration Date	6 months
Description	Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-Lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. BioAssay Systems EnzyFluo™ lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the lactate concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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