CD Diamine Oxidase Assay Kit

Catalog Number: AKBA251

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$1018

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Specifications

Product Information
Product Name	CD Diamine Oxidase Assay Kit
Applications	For quantitative determination of diamine oxidase and evaluation of drug modulators.
Features	Fast and sensitive. Use of 10 µL sample. Linear detection range 0.5 to 6 U/L for 30 min reaction at 25°C. Convenient. The procedure involves adding a single working reagent, and reading the fluorescence at 0 and 30 minutes. Room temperature assay. No 37°C heater is needed. High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Detection Method	FL530/585nm
Sample Type	Serum, Plasma
Species	All
Assay Time	30 min
Limit of Detection	0.5 U/L
Expiration Date	6 months
Description	DIAMINE OXIDASE (DAO) also known as histaminase or amine oxidase (copper containing), is an enzyme involved in the metabolism, oxidation, and inactivation of histamine in animals. Highest content is observed in the digestive tract and placenta. An imbalance between histamine intake and the capacity for histamine degradation can lead to histamine intolerance (HIT). Measuring DAO activity in serum can be useful in diagnosing HIT.CD non-radioactive, fluorimetric DAO assay is based on the oxidation of putrescine to pyrroline plus NH3 and H2O2. The generated H2O2 is then used by HRP to oxidize a dye making it fluorescent. The increase in fluorescence at λex/em = 530/585 nm is directly proportional to the enzyme activity. • Enzyme-Activity-Assays.htm” target=”_blank”>Other Enzyme Activity Assay Kits • Metabolism.htm” target=”_blank”>Other Metabolism Assay Kits
Shipping Conditions	Shipping: RT
Storage	Storage: -20°C upon receipt

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