CD Farnesyltransferase Activity Assay Kit

Catalog Number: AKBA212

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$1077

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Specifications

Product Information
Product Name	CD Farnesyltransferase Activity Assay Kit
Applications	For quantitative determination of FTase enzyme activity in biological samples.
Features	Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Sensitive and accurate. Linear detection range 0.024 – 3.2 U/L FTase in a 384-well plate assay. High-throughput. Can be readily automated to assay thousands of samples per day.
Detection Method	FL340/550 nm
Sample Type	Biological Samples, Purified Enzyme
Species	All
Assay Time	60 min
Limit of Detection	0.024 U/L
Expiration Date	6 months
Description	FARNESYLTRANSFERASE (FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).
Shipping Conditions	Shipping: Ice
Storage	Storage: -20°C upon receipt.

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