CD Free Fatty Acid Assay Kit

Catalog Number: AKBA021

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$1407

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Specifications

Product Information
Product Name	CD Free Fatty Acid Assay Kit
Applications	For quantitative determination of fatty acid and evaluation of drug effects on its metabolism.
Features	Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 7 – 1000 µM, fluorimetric assay 7 – 100 µM fatty acid. Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day.
Detection Method	OD570nm, or FL530/585nm
Sample Type	Serum, plasma, urine, saliva, milk, cell cultures, food, agriculture etc
Species	All
Assay Time	30 min
Limit of Detection	7 µM
Expiration Date	6 months
Description	Fatty acids are aliphatic monocarboxylic acids that are ubiquitously found in animal or vegetable fat, oil, and wax. Fatty Acids play important roles in cellular synthesis, and energy metabolism and are implicated in diverse disorders such as diabetes mellitus, sudden infant death syndrome, and Reye Syndrome. CD method provides a simple, one-step, and high-throughput assay for measuring free fatty acids. In this assay, free fatty acids are enzymatically converted to acyl-CoA and subsequently to H2O2. The resulting H2O2 reacts with a specific dye to form a pink-colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the free fatty acid concentration in the sample.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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