CD Fumarase Assay Kit

Catalog Number: AKBA022

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$1118

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Specifications

Product Information
Product Name	CD Fumarase Assay Kit
Applications	For quantitative determination of fumarase enzyme activity.
Features	Fast and sensitive. Linear detection range: 0.4 to 70 U/L for 30 min reaction at 37°C. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Detection Method	OD565nm
Sample Type	Biological samples (e.g. plasma, serum, erythrocytes, tissue, and culture media)
Species	All
Assay Time	40 min
Limit of Detection	0.4 U/L
Expiration Date	6 months
Description	FUMARASE (or fumarate hydratase) (EC 4.2.1.2) is an enzyme that catalyzes the reversible hydration/dehydration reaction of fumarate to malate. Fumarase exists in two isoforms: a cytosolic and mitochondrial form. In the citric acid cycle, it facilitates a transition step in the production of energy in the form of NADH. Fumarase deficiency in humans results in early brain development problems and is characterized by poor feeding, hypotonia, failure to thrive, etc. BioAssay Systems’ non-radioactive, colorimetric fumarase assay is based on the reduction of the tetrazolium salt MTT in an NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Shipping Conditions	Shipping: On Ice
Storage	Storage: -20°C upon receipt

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